ArticleMethods in molecular biology (Clifton, N.J.)2012
Splint ligation of RNA with T4 DNA ligase.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 52 citations in OpenAlex.
- Generation of precise and accurate engineered circRNAs using enzymatic ligation.Nucleic acids research · 2026Article
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- Application of NMR to Large RNAs.Journal of molecular biology · 2025Review
- A DNA Scaffold Approach Facilitates 5' Labeling of the SARS-CoV-2 RNA Pseudoknot for Single-Molecule Förster Resonance Energy Transfer Investigation.Chemphyschem : a European journal of chemical physics and physical chemistry · 2025Article
- Use of synthetic circular RNA spike-ins (SynCRS) for normalization of circular RNA sequencing data.Nature protocols · 2025Review
- mRNA medicine: Recent progresses in chemical modification, design, and engineering.Nano research · 2024Article
- Advances in methods for tRNA sequencing and quantification.Trends in genetics : TIG · 2024Review
- Effective synthesis of circRNA via a thermostable T7 RNA polymerase variant as the catalyst.Frontiers in bioengineering and biotechnology · 2024Article
- Review
- Review
- The hnRNP C tetramer binds to CBC on mRNA and impedes PHAX recruitment for the classification of RNA polymerase II transcripts.Nucleic acids research · 2023Article
- Elucidating the Kinetic Mechanism of Human METTL16.Biochemistry · 2023Article
- The RNA ligation method using modified splint DNAs significantly improves the efficiency of circular RNA synthesis.Animal cells and systems · 2023Article
- Circular RNA: An emerging frontier in RNA therapeutic targets, RNA therapeutics, and mRNA vaccines.Journal of controlled release : official journal of the Controlled Release Society · 2022Review
- The design and synthesis of circular RNAs.Methods (San Diego, Calif.) · 2021Review
- Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles.Nature communications · 2021Article
- Strategies for Covalent Labeling of Long RNAs.Chembiochem : a European journal of chemical biology · 2021Review
- Challenges to optimizing RNA nanostructures for large scale production and controlled therapeutic properties.Nanomedicine (London, England) · 2020Article
- Artificial Circular RNA Sponges Targeting MicroRNAs as a Novel Tool in Molecular Biology.Molecular therapy. Nucleic acids · 2019Article
- Strategic labelling approaches for RNA single-molecule spectroscopy.RNA biology · 2019Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Splint ligation of RNA, whereby specific RNA molecules are ligated together, can be carried out using T4 DNA ligase and a bridging DNA oligonucleotide complementary to the RNAs. This method takes advantage of the property of T4 DNA ligase to join RNA molecules when they are in an RNA:DNA hybrid. Splint ligation is a useful tool for the introduction of modified nucleotides into RNA molecules, insertion of a radiolabel into a specific position within an RNA and for the assembly of smaller synthetic RNAs into longer RNA molecules. Such modifications enable a wide range of experiments to be carried out with the modified RNA including structural studies, co-immunoprecipitations, and the ability to map sites of RNA:RNA and RNA:protein interactions.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.