ArticleMethods in molecular biology (Clifton, N.J.)2012
End-labeling oligonucleotides with chemical tags after synthesis.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
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- The interplay between peptides and RNA is critical for protoribosome compartmentalization and stability.Nucleic acids research · 2024Article
- Molecular basis of CRX/DNA recognition and stoichiometry at the Ret4 response element.Structure (London, England : 1993) · 2024Article
- ATP-induced cross-linking of a biomolecular condensate.Biophysical journal · 2024Article
- Unrepaired base excision repair intermediates in template DNA strands trigger replication fork collapse and PARP inhibitor sensitivity.The EMBO journal · 2023Article
- ATP-induced crosslinking of a biomolecular condensate.bioRxiv : the preprint server for biology · 2023Article
- Site-Specific Covalent Labeling of DNA Substrates by an RNA Transglycosylase.Journal of the American Chemical Society · 2023Article
- HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins.Nature communications · 2022Article
- NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin.Nucleic acids research · 2022Article
- Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2.Nature communications · 2021Article
- End-labeling-based electrochemical strategy for detection of adenine methylation in nucleic acid by differential pulse voltammetry.Mikrochimica acta · 2021Article
- Substrate discrimination and quality control require each catalytic activity of TRAMP and the nuclear RNA exosome.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Getting DNA and RNA out of the dark with 2CNqA: a bright adenine analogue and interbase FRET donor.Nucleic acids research · 2020Article
- The Sox2 transcription factor binds RNA.Nature communications · 2020Article
- Article
- The glucocorticoid receptor DNA-binding domain recognizes RNA hairpin structures with high affinity.Nucleic acids research · 2019Article
- The Growing Development of DNA Nanostructures for Potential Healthcare-Related Applications.Advanced healthcare materials · 2019Review
- Template-directed RNA polymerization and enhanced ribozyme catalysis inside membraneless compartments formed by coacervates.Nature communications · 2019Article
- Ensemble and single-molecule FRET studies of protein synthesis.Methods (San Diego, Calif.) · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Many experimental strategies for determining nucleic acid function require labeling the nucleic acid with radioisotopes or a chemical tag. Labels enable nucleic acid detection, yield information about its state, and can serve as a handle by which the nucleic acid and associated factors can be purified from a mixture. Radioactive phosphate is commonly added to the 5' or 3' end of an oligonucleotide post synthesis using enzyme-catalyzed reactions. In contrast, chemical tags are usually added during synthesis or using reactive groups that are incorporated during synthesis. Here, we present protocols for post-synthetic conjugation of chemical tags to unmodified RNA or DNA oligonucleotides. The approach can be used to attach fluorescent dyes and biotin groups to oligonucleotides and to immobilize oligonucleotides to a solid support. Oligonucleotides tagged with fluorescent dyes are readily detected in both gel- and plate reader-based assays, while biotin- or resin-conjugated oligonucleotides are useful tools for affinity purification. Fluorescent end-labeling provides several advantages over radioactive labeling, reducing radioactivity-associated hazards and yielding a labeled molecule that does not decay while providing the sensitivity required for many procedures.
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