ArticleMolecular & cellular proteomics : MCP2013
The proteome of copper, iron, zinc, and manganese micronutrient deficiency in Chlamydomonas reinhardtii.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 105 citations in OpenAlex.
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- Unlocking Phytoplankton Metallomes with Comparative Analysis of Metal Quotas, Quantitative Proteomics, and Inferred Metalloproteomes.Environmental science & technology · 2025Article
- Too dim, too bright, and just right: Systems analysis of the Chlamydomonas diurnal program under limiting and excess light.The Plant cell · 2025Article
- The Elements of Life, Photosynthesis and Genomics.Journal of molecular biology · 2025Review
- Chlamydomonas cells transition through distinct Fe nutrition stages within 48 h of transfer to Fe-free medium.Photosynthesis research · 2024Article
- Iron rescues glucose-mediated photosynthesis repression during lipid accumulation in the green alga Chromochloris zofingiensis.Nature communications · 2024Article
- Dietary zinc requirement of juvenile stinging catfishHeliyon · 2024Article
- Review
- Distinct function of Chlamydomonas CTRA-CTR transporters in Cu assimilation and intracellular mobilization.Metallomics : integrated biometal science · 2024Article
- Effects of Dietary Zinc on Growth, Haematological Indices, Digestive Enzyme Activity, Tissue Mineralization, Antioxidant and Immune Status of Fingerling Heteropneustes fossilis.Biological trace element research · 2024Article
- Review
- Two related families of metal transferases, ZNG1 and ZNG2, are involved in acclimation to poor Zn nutrition in Arabidopsis.Frontiers in plant science · 2023Article
- Article
- Whole-genome scanning reveals environmental selection mechanisms that shape diversity in populations of the epipelagic diatom Chaetoceros.PLoS biology · 2022Article
- Proteomic and Genomic Studies of Micronutrient Deficiency and Toxicity in Plants.Plants (Basel, Switzerland) · 2022Review
- Copper Requirement and Acquisition by Marine Microalgae.Microorganisms · 2022Review
- Grazing rest during spring regreening period promotes the ecological restoration of degraded alpine meadow vegetation through enhanced plant photosynthesis and respiration.Frontiers in plant science · 2022Article
- Phenotypic changes in microalgae at acidic pH mediate their tolerance to higher concentrations of transition metals.Current research in microbial sciences · 2021Article
- COG0523 proteins: a functionally diverse family of transition metal-regulated G3E P-loop GTP hydrolases from bacteria to man.Metallomics : integrated biometal science · 2021Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
Trace metals such as copper, iron, zinc, and manganese play important roles in several biochemical processes, including respiration and photosynthesis. Using a label-free, quantitative proteomics strategy (MS(E)), we examined the effect of deficiencies in these micronutrients on the soluble proteome of Chlamydomonas reinhardtii. We quantified >10(3) proteins with abundances within a dynamic range of 3 to 4 orders of magnitude and demonstrated statistically significant changes in ~200 proteins in each metal-deficient growth condition relative to nutrient-replete media. Through analysis of Pearson's coefficient, we also examined the correlation between protein abundance and transcript abundance (as determined via RNA-Seq analysis) and found moderate correlations under all nutritional states. Interestingly, in a subset of transcripts known to significantly change in abundance in metal-replete and metal-deficient conditions, the correlation to protein abundance is much stronger. Examples of new discoveries highlighted in this work include the accumulation of O(2) labile, anaerobiosis-related enzymes (Hyd1, Pfr1, and Hcp2) in copper-deficient cells; co-variation of Cgl78/Ycf54 and coprogen oxidase; the loss of various stromal and lumenal photosynthesis-related proteins, including plastocyanin, in iron-limited cells; a large accumulation (from undetectable amounts to over 1,000 zmol/cell) of two COG0523 domain-containing proteins in zinc-deficient cells; and the preservation of photosynthesis proteins in manganese-deficient cells despite known losses in photosynthetic function in this condition.
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