ArticleTransgenic research2013
Characterising microbial protein test substances and establishing their equivalence with plant-produced proteins for use in risk assessments of transgenic crops.
Article in Transgenic research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed.
- Scientific assessment of the 2024 post-market environmental monitoring report on the cultivation of genetically modified maize MON 810 in the EU.EFSA journal. European Food Safety Authority · 2026Article
- Article
- Safety assessment of membrane-associated protoporphyrinogen IX oxidase fromGM crops & food · 2025Article
- Assessment of the 2023 post-market environmental monitoring report on the cultivation of genetically modified maize MON 810 in the EU.EFSA journal. European Food Safety Authority · 2025Article
- Assessment of the 2022 post-market environmental monitoring report on the cultivation of genetically modified maize MON 810 in the EU.EFSA journal. European Food Safety Authority · 2024Article
- Food and feed safety of the Bacillus thuringiensis derived protein Vpb4Da2, a novel protein for control of western corn rootworm.PloS one · 2022Article
- Assessment of the 2019 post-market environmental monitoring report on the cultivation of genetically modified maize MON 810 in the EU.EFSA journal. European Food Safety Authority · 2021Article
- Assessment of the 2018 post-market environmental monitoring report on the cultivation of genetically modified maize MON 810 in the EU.EFSA journal. European Food Safety Authority · 2020Article
- Meeting technical challenges for protein characterization and surrogate equivalence studies that resulted from insecticidal protein co-expression in maize event MZIR098.Transgenic research · 2020Article
- Sublethal Endpoints in Non-target Organism Testing for Insect-Active GE Crops.Frontiers in bioengineering and biotechnology · 2020Review
- Assessment of the 2017 post-market environmental monitoring report on the cultivation of genetically modified maize MON 810.EFSA journal. European Food Safety Authority · 2019Article
- Annual post-market environmental monitoring report on the cultivation of genetically modified maize MON 810 in 2016.EFSA journal. European Food Safety Authority · 2018Article
- Quality of laboratory studies assessing effects of Bt-proteins on non-target organisms: minimal criteria for acceptability.Transgenic research · 2016Review
- Impact of Spodoptera frugiperda neonate pretreatment conditions on Vip3Aa19 insecticidal protein activity and laboratory bioassay variation.Pest management science · 2016Article
- Transgenic maize event TC1507: Global status of food, feed, and environmental safety.GM crops & food · 2015Review
- Chronic Responses of Daphnia magna Under Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt-Maize Hybrid and its Conventional Near-Isoline.Journal of toxicology and environmental health. Part A · 2015Article
- Nontarget organism effects tests on eCry3.1Ab and their application to the ecological risk assessment for cultivation of Event 5307 maize.Transgenic research · 2014Article
- Protection goals in environmental risk assessment: a practical approach.Transgenic research · 2014Article
- Use of a pollen-based diet to expose the ladybird beetle Propylea japonica to insecticidal proteins.PloS one · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most commercial transgenic crops are genetically engineered to produce new proteins. Studies to assess the risks to human and animal health, and to the environment, from the use of these crops require grams of the transgenic proteins. It is often extremely difficult to produce sufficient purified transgenic protein from the crop. Nevertheless, ample protein of acceptable purity may be produced by over-expressing the protein in microbes such as Escherichia coli. When using microbial proteins in a study for risk assessment, it is essential that their suitability as surrogates for the plant-produced transgenic proteins is established; that is, the proteins are equivalent for the purposes of the study. Equivalence does not imply that the plant and microbial proteins are identical, but that the microbial protein is sufficiently similar biochemically and functionally to the plant protein such that studies using the microbial protein provide reliable information for risk assessment of the transgenic crop. Equivalence is a judgement based on a weight of evidence from comparisons of relevant properties of the microbial and plant proteins, including activity, molecular weight, amino acid sequence, glycosylation and immuno-reactivity. We describe a typical set of methods used to compare proteins in regulatory risk assessments for transgenic crops, and discuss how risk assessors may use comparisons of proteins to judge equivalence.
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Registered trials
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