ArticleMolecular biology reports2013
Zn(2+) and L-isoleucine induce the expressions of porcine β-defensins in IPEC-J2 cells.
Article in Molecular biology reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Piggybacking on nature: exploring the multifaceted world of porcine β-defensins.Veterinary research · 2025Review
- BCAAs acutely drive glucose dysregulation and insulin resistance: role of AgRP neurons.Nutrition & diabetes · 2024Article
- Mechanisms and regulation of defensins in host defense.Signal transduction and targeted therapy · 2023Review
- Preventing Respiratory Viral Diseases with Antimicrobial Peptide Master Regulators in the Lung Airway Habitat.Clinics and practice · 2023Review
- Histones of Neutrophil Extracellular Traps Induce CD11b Expression in Brain Pericytes Via Dectin-1 after Traumatic Brain Injury.Neuroscience bulletin · 2022Article
- Porcine and Chicken Intestinal Epithelial Cell Models for Screening Phytogenic Feed Additives-Chances and Limitations in Use as Alternatives to Feeding Trials.Microorganisms · 2022Review
- Immunomodulatory effects of new phytotherapy on human macrophages and TLR4- and TLR7/8-mediated viral-like inflammation in mice.Frontiers in medicine · 2022Article
- Amino acids and the regulation of oxidative stress and immune function in dairy cattle.Journal of animal science · 2020Article
- Expression and Functional Characterization of a Novel Antimicrobial Peptide: Human Beta-Defensin 118.BioMed research international · 2020Article
- Recent Progress on Branched-Chain Amino Acids in Obesity, Diabetes, and Beyond.Endocrinology and metabolism (Seoul, Korea) · 2019Review
- β-Defensin 129 Attenuates Bacterial Endotoxin-Induced Inflammation and Intestinal Epithelial Cell Apoptosis.Frontiers in immunology · 2019Article
- Leucine modulates the IPEC-J2 cell proteome associated with cell proliferation, metabolism and phagocytosis.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2018Article
- l-Isoleucine Administration Alleviates Rotavirus Infection and Immune Response in the Weaned Piglet Model.Frontiers in immunology · 2018Article
- Novel metabolic and physiological functions of branched chain amino acids: a review.Journal of animal science and biotechnology · 2017Review
- Leucine increases mucin 2 and occludin production in LS174T cells partially via PI3K-Akt-mTOR pathway.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2016Article
- Induction of porcine host defense peptide gene expression by short-chain fatty acids and their analogs.PloS one · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The β-defensins, expressed in epithelial cells of multiple tissues including intestine, play a critical role in the mammalian innate immunity. However, it is little known about the role of functional nutrients in the regulation of porcine β-defensins' expressions in intestinal epithelial cells. The present study was conducted to determine the hypothesis that zinc and L-isoleucine regulate the expressions of porcine β-defensins in IPEC-J2 cells. Cells were cultured in DMEM/F12 medium containing supplemental 0-500 μg/mL L-isoleucine or 0-500 μmol/mL zinc sulfate that was used to increase the concentration of Zn(2+) in the medium. At 12 h after the treatment by the appropriate concentrations of L-isoleucine or Zn(2+), the mRNA and protein expressions of porcine β-defensin 1, 2 and 3 were increased (P < 0.05), and reached their maximum after treatment with 25 or 100 μmol/mL zinc sulfate and 25 or 50 μg/mL isoleucine (P < 0.05). These results suggested that both Zn(2+) and L-isoleucine could induce β-defensins' expressions in porcine intestinal epithelial cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.