ArticlePlant physiology2012
Arabidopsis plastid AMOS1/EGY1 integrates abscisic acid signaling to regulate global gene expression response to ammonium stress.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 126 citations in OpenAlex.
- Article
- The Involvement of thePlants (Basel, Switzerland) · 2025Article
- Overexpression of pxAlaAT3 in Populus × xiaohei alleviates root growth inhibition under ammonium nitrogen stress.Plant cell reports · 2025Article
- Phenotype Assessment and Putative Mechanisms of Ammonium Toxicity to Plants.International journal of molecular sciences · 2025Review
- Mapping and functional characterization of the golden fruit 1 (gf1) in melon (Cucumis melo L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- SALT OVERLY SENSITIVE2 and AMMONIUM TRANSPORTER1;1 contribute to plant salt tolerance by maintaining ammonium uptake.The Plant cell · 2025Article
- Site-2 protease Sll0528 interacts with RbcR to regulate carbon/nitrogen homeostasis in the cyanobacteriumFrontiers in microbiology · 2025Article
- Protein degrons and degradation: Exploring substrate recognition and pathway selection in plants.The Plant cell · 2024Review
- Physiology, genomics, and evolutionary aspects of desert plants.Journal of advanced research · 2024Review
- EvaluatingFrontiers in plant science · 2024Article
- Rare occurrence of cryptic 5' splice sites by downstream 3' splice site/exon boundary mutations in a heavy-ion-inducedFrontiers in plant science · 2024Article
- Rewiring of primary metabolism for ammonium recycling under short-term low COFrontiers in plant science · 2024Article
- S2P2-the chloroplast-located intramembrane protease and its impact on the stoichiometry and functioning of the photosynthetic apparatus ofFrontiers in plant science · 2024Article
- Plastid-localized amino acid metabolism coordinates rice ammonium tolerance and nitrogen use efficiency.Nature plants · 2023Article
- Chloroplast metalloproteinase SlL2 reduces the thermotolerance of tomato by decreasing the content of SlCDJ1.Protoplasma · 2023Article
- Analysis of Potato Physiological and Molecular Adaptation in Response to Different Water and Nitrogen Combined Regimes.Plants (Basel, Switzerland) · 2023Article
- Site-2 Protease Slr1821 Regulates Carbon/Nitrogen Homeostasis during Ammonium Stress Acclimation in CyanobacteriumInternational journal of molecular sciences · 2023Article
- Integrated comparative transcriptome and physiological analysis reveals the metabolic responses underlying genotype variations in NHFrontiers in plant science · 2023Article
- TheBiology · 2022Article
- Abscisic acid influences ammonium transport via regulation of kinase CIPK23 and ammonium transporters.Plant physiology · 2022Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ammonium (NH(4)(+)) is a ubiquitous intermediate of nitrogen metabolism but is notorious for its toxic effects on most organisms. Extensive studies of the underlying mechanisms of NH(4)(+) toxicity have been reported in plants, but it is poorly understood how plants acclimate to high levels of NH(4)(+). Here, we identified an Arabidopsis (Arabidopsis thaliana) mutant, ammonium overly sensitive1 (amos1), that displays severe chlorosis under NH(4)(+) stress. Map-based cloning shows amos1 to carry a mutation in EGY1 (for ethylene-dependent, gravitropism-deficient, and yellow-green-like protein1), which encodes a plastid metalloprotease. Transcriptomic analysis reveals that among the genes activated in response to NH(4)(+), 90% are regulated dependent on AMOS1/EGY1. Furthermore, 63% of AMOS1/EGY1-dependent NH(4)(+)-activated genes contain an ACGTG motif in their promoter region, a core motif of abscisic acid (ABA)-responsive elements. Consistent with this, our physiological, pharmacological, transcriptomic, and genetic data show that ABA signaling is a critical, but not the sole, downstream component of the AMOS1/EGY1-dependent pathway that regulates the expression of NH(4)(+)-responsive genes and maintains chloroplast functionality under NH(4)(+) stress. Importantly, abi4 mutants defective in ABA-dependent and retrograde signaling, but not ABA-deficient mutants, mimic leaf NH(4)(+) hypersensitivity of amos1. In summary, our findings suggest that an NH(4)(+)-responsive plastid retrograde pathway, which depends on AMOS1/EGY1 function and integrates with ABA signaling, is required for the regulation of expression of NH(4)(+)-responsive genes that maintain chloroplast integrity in the presence of high NH(4)(+) levels.
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