ArticleThe Plant cell2012
Agrobacterium may delay plant nonhomologous end-joining DNA repair via XRCC4 to favor T-DNA integration.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Arabidopsis DNA repair mutants can integrate Agrobacterium T-DNA into the plant genome.The New phytologist · 2026Article
- Transformation and regeneration of DNA polymerase Θ mutant rice plants.Plant direct · 2023Article
- Distinct mechanisms for genomic attachment of the 5' and 3' ends of Agrobacterium T-DNA in plants.Nature plants · 2022Article
- Genetic factors governing bacterial virulence and host plant susceptibility duringAdvances in genetics · 2022Review
- Characterization of T-Circles and Their Formation Reveal Similarities toFrontiers in plant science · 2022Article
- Plant DNA Repair andInternational journal of molecular sciences · 2021Review
- Agrobacterium T-DNA integration in somatic cells does not require the activity of DNA polymerase θ.The New phytologist · 2021Article
- Review
- Article
- Overexpression of VIRE2-INTERACTING PROTEIN2 in Arabidopsis regulates genes involved in Agrobacterium-mediated plant transformation and abiotic stresses.Scientific reports · 2019Article
- Pathways of DNA Transfer to Plants fromAnnual review of phytopathology · 2019Article
- Screening internal controls for expression analyses involving numerous treatments by combining statistical methods with reference gene selection tools.Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Plant reference genes for development and stress response studies.Journal of biosciences · 2018Review
- Agrobacterium-mediated plant transformation: biology and applications.The arabidopsis book · 2017Article
- The non-homologous end-joining pathway is involved in stable transformation in rice.Frontiers in plant science · 2014Review
- The roles of bacterial and host plant factors in Agrobacterium-mediated genetic transformation.The International journal of developmental biology · 2013Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium tumefaciens is a soilborne pathogen that causes crown gall disease in many dicotyledonous plants by transfer of a portion of its tumor-inducing plasmid (T-DNA) into the plant genome. Several plant factors that play a role in Agrobacterium attachment to plant cells and transport of T-DNA to the nucleus have been identified, but the T-DNA integration step during transformation is poorly understood and has been proposed to occur via nonhomologous end-joining (NHEJ)-mediated double-strand DNA break (DSB) repair. Here, we report a negative role of X-ray cross complementation group4 (XRCC4), one of the key proteins required for NHEJ, in Agrobacterium T-DNA integration. Downregulation of XRCC4 in Arabidopsis and Nicotiana benthamiana increased stable transformation due to increased T-DNA integration. Overexpression of XRCC4 in Arabidopsis decreased stable transformation due to decreased T-DNA integration. Interestingly, XRCC4 directly interacted with Agrobacterium protein VirE2 in a yeast two-hybrid system and in planta. VirE2-expressing Arabidopsis plants were more susceptible to the DNA damaging chemical bleomycin and showed increased stable transformation. We hypothesize that VirE2 titrates or excludes active XRCC4 protein available for DSB repair, thus delaying the closure of DSBs in the chromosome, providing greater opportunity for T-DNA to integrate.
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