ArticleEukaryotic cell2012
Intracellular NADPH levels affect the oligomeric state of the glucose 6-phosphate dehydrogenase.
Article in Eukaryotic cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Genetic and Physiological Characterization of the Pentose Phosphate Pathway in the YeastInternational journal of molecular sciences · 2025Article
- The Key Enzymes of Carbon Metabolism and the Glutathione Antioxidant System ProtectJournal of fungi (Basel, Switzerland) · 2024Article
- Chemiosmotic nutrient transport in synthetic cells powered by electrogenic antiport coupled to decarboxylation.Nature communications · 2024Article
- The Role of Glucose-6-phosphate Dehydrogenase in the Wine YeastInternational journal of molecular sciences · 2024Article
- Cellular Redox Metabolism Is Modulated by the Distinct Localization of Cyclic Nucleotide Phosphodiesterase 5A Isoforms.International journal of molecular sciences · 2022Article
- Monitoring Intracellular Metabolite Dynamics inMetabolites · 2022Article
- Precursor Quantitation Methods for Next Generation Food Production.Frontiers in bioengineering and biotechnology · 2022Review
- Review
- Metabolic Remodeling during Long-Lasting Cultivation of theMicroorganisms · 2020Article
- Use of the KlADH3 promoter for the quantitative production of the murine PDE5A isoforms in the yeast Kluyveromyces lactis.Microbial cell factories · 2017Article
- TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells.PloS one · 2017Article
- NADPH-generating systems in bacteria and archaea.Frontiers in microbiology · 2015Review
- Effect of oxygenation and temperature on glucose-xylose fermentation in Kluyveromyces marxianus CBS712 strain.Microbial cell factories · 2014Article
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6 authors.
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Abstract
In the yeast Kluyveromyces lactis, glucose 6-phosphate dehydrogenase (G6PDH) is detected as two differently migrating forms on native polyacrylamide gels. The pivotal metabolic role of G6PDH in K. lactis led us to investigate the mechanism controlling the two activities in respiratory and fermentative mutant strains. An extensive analysis of these mutants showed that the NAD(+)(H)/NADP(+)(H)-dependent cytosolic alcohol (ADH) and aldehyde (ALD) dehydrogenase balance affects the expression of the G6PDH activity pattern. Under fermentative/ethanol growth conditions, the concomitant activation of ADH and ALD activities led to cytosolic accumulation of NADPH, triggering an alteration in the oligomeric state of the G6PDH caused by displacement/release of the structural NADP(+) bound to each subunit of the enzyme. The new oligomeric G6PDH form with faster-migrating properties increases as a consequence of intracellular redox unbalance/NADPH accumulation, which inhibits G6PDH activity in vivo. The appearance of a new G6PDH-specific activity band, following incubation of Saccharomyces cerevisiae and human cellular extracts with NADP(+), also suggests that a regulatory mechanism of this activity through NADPH accumulation is highly conserved among eukaryotes.
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