ArticleRNA biology2012
Expression and regulation of mouse selenoprotein P transcript variants differing in non-coding RNA.
Article in RNA biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Functional Annotation of MicroRNAs Using Existing Resources.Methods in molecular biology (Clifton, N.J.) · 2022Article
- MiR-7 in Cancer Development.Biomedicines · 2021Review
- miR760 regulates ATXN1 levels via interaction with its 5' untranslated region.Genes & development · 2020Article
- MicroRNA-7: expression and function in brain physiological and pathological processes.Cell & bioscience · 2020Review
- Regulation of miR-34 Family in Neuronal Development.Molecular neurobiology · 2018Article
- Increased Δ133p53 mRNA in lung carcinoma corresponds with reduction of p21 expression.Molecular medicine reports · 2017Article
- Human selenoprotein P and S variant mRNAs with different numbers of SECIS elements and inferences from mutant mice of the roles of multiple SECIS elements.Open biology · 2016Article
- Umbilical cord blood and placental mercury, selenium and selenoprotein expression in relation to maternal fish consumption.Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) · 2015Article
- Regulation of selenocysteine incorporation into the selenium transport protein, selenoprotein P.The Journal of biological chemistry · 2014Article
- MiR-103a-3p targets the 5' UTR of GPRC5A in pancreatic cells.RNA (New York, N.Y.) · 2014Article
- Brain microRNAs and insights into biological functions and therapeutic potential of brain enriched miRNA-128.Molecular cancer · 2014Review
- The molecular biology of selenocysteine.Biomolecular concepts · 2013Review
- Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Selenoprotein P (Sepp1), a glycoprotein rich in selenium, is thought to function in selenium transport throughout the body. The sepp1 gene locus potentially produces three alternative transcripts that differ only in their 5' untranslated regions (5'UTRs) and not in their protein coding regions, as indicated by transcript information in genomic databases. Here we investigated the distribution, relative expression, and biological significance of these transcript variants. We confirmed the expression of Sepp1 transcript variants using PCR and sequencing. Using 5'-RACE, we identified multiple 5'-termini upstream from three different splice donor sites, and a single splice acceptor site for exon 2. We found regional and temporal changes in variant expression in select adult and neonate murine tissue and brain regions. Distribution of variants in heart and kidney varied with stage of development. Notably, the Sepp1b variant was localized specifically to the hippocampus in brain. Targeted silencing of individual variants using RNAi demonstrated the biological importance for all transcript variants in cell viability. Additionally, we determined that the Sepp1b variant is a specific target for the miR-7 microRNA by means of its unique 5'UTR structure. Our results emphasize the importance of non-coding transcript variations as a regulatory means for Sepp1 expression in different tissues and stages of development. The presence of a variant localized in the hippocampus and regulated by a microRNA may have implications for the known deficits in synaptic function caused by genetic deletion of Sepp1.
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