ArticleCurrent biology : CB2012
Adhesion regulates MAP kinase/ternary complex factor exchange to control a proliferative transcriptional switch.
Article in Current biology : CB, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Multi-omics characterization of chronic social defeat stress recall-activated nuclei in Arc-GFP mice.Molecular psychiatry · 2026Article
- Mechanical regulation of chromatin and transcription.Nature reviews. Genetics · 2022Review
- Single-molecule tracking (SMT) and localization of SRF and MRTF transcription factors during neuronal stimulation and differentiation.Open biology · 2022Article
- ELK1 Promotes Epithelial-Mesenchymal Transition and the Progression of Lung Adenocarcinoma by Upregulating B7-H3.Oxidative medicine and cellular longevity · 2021Article
- Crosstalk between mechanotransduction and metabolism.Nature reviews. Molecular cell biology · 2021Review
- Material microenvironmental properties couple to induce distinct transcriptional programs in mammalian stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Cellular adaptation to biomechanical stress across length scales in tissue homeostasis and disease.Seminars in cell & developmental biology · 2017Review
- SRF Co-factors Control the Balance between Cell Proliferation and Contractility.Molecular cell · 2016Article
- Opposing effects of Elk-1 multisite phosphorylation shape its response to ERK activation.Science (New York, N.Y.) · 2016Article
- Dynamic transcription factor activity networks in response to independently altered mechanical and adhesive microenvironmental cues.Integrative biology : quantitative biosciences from nano to macro · 2016Article
- Review
- XRP44X, an Inhibitor of Ras/Erk Activation of the Transcription Factor Elk3, Inhibits Tumour Growth and Metastasis in Mice.PloS one · 2016Article
- Two hydrophobic residues can determine the specificity of mitogen-activated protein kinase docking interactions.The Journal of biological chemistry · 2015Article
- Mechanical Forces and Growth in Animal Tissues.Cold Spring Harbor perspectives in biology · 2015Review
- Forms, forces, and stem cell fate.Current opinion in cell biology · 2014Review
- Bioengineering paradigms for cell migration in confined microenvironments.Current opinion in cell biology · 2014Review
- The oncogenic MicroRNA Hsa-miR-155-5p targets the transcription factor ELK3 and links it to the hypoxia response.PloS one · 2014Article
- Article
- From tissue mechanics to transcription factors.Differentiation; research in biological diversity · 2013Review
- Role of the extracellular matrix in regulating stem cell fate.Nature reviews. Molecular cell biology · 2013Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe ternary complex factors (TCFs; Elk1, Net, and Sap-1) are growth factor-responsive transcription cofactors of serum response factor (SRF) and are activated by MAP kinase (MAPK) phosphorylation to regulate immediate early gene transcription. Although cell adhesion also can regulate immediate early genes and proliferation, the mechanism for this effect has remained unexplored.
resultsRestricting adhesion and spreading of G(0)-synchronized cells on substrates with decreasing size of micropatterned islands of fibronectin suppressed serum-induced immediate early gene expression and S phase entry. Knockdown of Sap-1 decreased expression of the immediate early genes egr1 and fos and subsequent proliferation normally present with high adhesion, whereas knockdown of Net rescued egr1 and fos expression and proliferation normally suppressed by low adhesion. Chromatin immunoprecipitation studies showed increased occupancy of egr1 and fos promoters by Sap-1 with high adhesion, whereas low adhesion increased Net occupancy. This switch in TCF promoter binding was regulated by an adhesion-mediated switch in MAPK activity. Increasing adhesion enhanced serum-induced JNK activity while suppressing p38 activity, leading to increased Sap-1 phosphorylation and Net dephosphorylation, and switching Net with Sap-1 at egr1 and fos promoters to support proliferation. Microarray studies confirmed this switch in TCF regulation of proliferative genes and uncovered novel gene targets and functions coregulated by Sap-1 and Net.
conclusionsThese data demonstrate a key role for the TCFs in adhesion-induced transcription and proliferation and reveal a novel MAPK/TCF transcriptional switch that controls this process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.