ArticleJournal of biomechanics2013
Depth-dependent anisotropy of the micromechanical properties of the extracellular and pericellular matrices of articular cartilage evaluated via atomic force microscopy.
Article in Journal of biomechanics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Alteration in cartilage matrix stiffness as an indicator and modulator of osteoarthritis.Bioscience reports · 2024Pooled it
- Understanding sub-cellular mechanochemical correlations of the neonatal extrahepatic bile duct extracellular matrix by atomic force microscope-Infrared spectroscopy.Analytica chimica acta · 2026Article
- TheMaterials today. Bio · 2026Review
- Viscoelastic recovery time of chondrocytes from monolayer and alginate cultures.bioRxiv : the preprint server for biology · 2026Article
- Innovations in collagen-network remodeling and extracellular matrix mechanics: toward a new era in articular cartilage repair.Frontiers in bioengineering and biotechnology · 2025Review
- Maternal high-fat high-sugar diet impairs bone quality and strength but not cartilage in aging mice.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2025Article
- Depth-Dependent Strain Model (1D) for Anisotropic Fibrils in Articular Cartilage.Materials (Basel, Switzerland) · 2024Article
- Pericellular Matrix Formation and Atomic Force Microscopy of Single Primary Human Chondrocytes Cultured in Alginate Microgels.Advanced biology · 2024Article
- HDAC6 inhibition regulates substrate stiffness-mediated inflammation signaling in chondrocytes.Acta biochimica et biophysica Sinica · 2023Article
- Articular Cartilage-From Basic Science Structural Imaging to Non-Invasive Clinical Quantitative Molecular Functional Information for AI Classification and Prediction.International journal of molecular sciences · 2023Review
- Biomechanics of Chondrocytes and Chondrons in Healthy Conditions and Osteoarthritis: A Review of the Mechanical Characterisations at the Microscale.Biomedicines · 2023Review
- Atomic Force Microscopy Micro-Indentation Methods for Determining the Elastic Modulus of Murine Articular Cartilage.Sensors (Basel, Switzerland) · 2023Article
- Determining Spatial Variability of Elastic Properties for Biological Samples Using AFM.Micromachines · 2023Review
- Targeting cell-matrix interface mechanobiology by integrating AFM with fluorescence microscopy.Progress in biophysics and molecular biology · 2022Review
- Metabolomic Profiling and Mechanotransduction of Single Chondrocytes Encapsulated in Alginate Microgels.Cells · 2022Article
- Material properties and strain distribution patterns of bovine growth plate cartilage vary with anatomic location and depth.Journal of biomechanics · 2022Article
- Regulation of FGF-2, FGF-18 and Transcription Factor Activity by Perlecan in the Maturational Development of Transitional Rudiment and Growth Plate Cartilages and in the Maintenance of Permanent Cartilage Homeostasis.International journal of molecular sciences · 2022Review
- Substrate stiffness-dependent regulatory volume decrease and calcium signaling in chondrocytes.Acta biochimica et biophysica Sinica · 2022Article
- A dysfunctional TRPV4-GSK3β pathway prevents osteoarthritic chondrocytes from sensing changes in extracellular matrix viscoelasticity.Nature biomedical engineering · 2021Article
- Impact of perlecan, a core component of basement membrane, on regeneration of cartilaginous tissues.Acta biomaterialia · 2021Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The extracellular matrix (ECM) of articular cartilage is structurally and mechanically inhomogeneous and anisotropic, exhibiting variations in composition, collagen fiber architecture, and pericellular matrix (PCM) morphology among the different zones (superficial, middle, and deep). Joint loading exposes chondrocytes to a complex biomechanical environment, as the microscale mechanical environment of the chondrocyte depends on the relative properties of its PCM and local ECM. ECM anisotropy and chondrocyte deformation are influenced by the split-line direction, the preferred collagen fiber orientation parallel to the articular surface. While previous studies have demonstrated that cartilage macroscale properties vary with depth and the direction of loading relative to the split-line direction, the potential anisotropic behavior of the ECM and PCM at the microscale has yet to be examined. The goal of this study was to characterize the depth and directional dependence of the microscale biomechanical properties of porcine cartilage ECM and PCM in situ. Cartilage was cryosectioned to generate samples oriented parallel and perpendicular to the split-line direction and normal to the articular surface. Atomic force microscopy (AFM)-based stiffness mapping was utilized to measure ECM and PCM microscale elastic properties in all three directions within each zone. Distinct anisotropy in ECM elastic moduli was observed in the superficial and deep zones, while the middle zone exhibited subtle anisotropy. PCM elastic moduli exhibited zonal uniformity with depth and directional dependence when pooled across the zones. These findings provide new evidence for mechanical inhomogeneity and anisotropy at the microscale in articular cartilage.
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Registered trials
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