ArticleBMC research notes2012
Cloning and identification of novel hydrolase genes from a dairy cow rumen metagenomic library and characterization of a cellulase gene.
Article in BMC research notes, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 70 citations in OpenAlex.
- Auxin biosynthesis and signaling drive virulence and plant adaptation in Dickeya dadantii.PLoS pathogens · 2026Article
- Multi-omics revealed the mechanism of feed efficiency in sheep by the combined action of the host and rumen microbiota.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- Enhanced crystalline cellulose degradation by a novel metagenome-derived cellulase enzyme.Scientific reports · 2024Article
- Potential of the quorum-quenching and plant-growth promoting halotolerant Bacillus toyonensis AA1EC1 as biocontrol agent.Microbial biotechnology · 2024Article
- Exploring the microbial diversity and characterization of cellulase and hemicellulase genes in goat rumen: a metagenomic approach.BMC biotechnology · 2023Article
- Role of metagenomics in prospecting novel endoglucanases, accentuating functional metagenomics approach in second-generation biofuel production: a review.Biomass conversion and biorefinery · 2023Review
- Starch and Cellulose Degradation in the Rumen and Applications of Metagenomics on Ruminal Microorganisms.Animals : an open access journal from MDPI · 2022Review
- Cloning of cellulase gene using metagenomic approach of soils collected from Wadi El Natrun, an extremophilic desert valley in Egypt.Journal, genetic engineering & biotechnology · 2022Article
- Cellulolytic thermophilic microorganisms in white biotechnology: a review.Folia microbiologica · 2020Review
- A Novel VIII Carboxylesterase with High Hydrolytic Activity Against Ampicillin from a Soil Metagenomic Library.Molecular biotechnology · 2019Article
- Identification and characterization of an Endo-glucanase secreted from cellulolytic Escherichia coli ZH-4.BMC biotechnology · 2019Article
- Biochemical Characteristics of Microbial Enzymes and Their Significance from Industrial Perspectives.Molecular biotechnology · 2019Review
- Metagenomics in ophthalmology: current findings and future prospectives.BMJ open ophthalmology · 2019Review
- IndigenousFrontiers in microbiology · 2018Article
- Genetics, Molecular, and Proteomics Advances in Filamentous Fungi.Current microbiology · 2017Review
- Isolation and characterization of a novel glycosyl hydrolase family 74 (GH74) cellulase from the black goat rumen metagenomic library.Folia microbiologica · 2017Article
- A Metagenomic Advance for the Cloning and Characterization of a Cellulase from Red Rice Crop Residues.Molecules (Basel, Switzerland) · 2016Article
- Chemical stability of a cold-active cellulase with high tolerance toward surfactants and chaotropic agent.Biotechnology reports (Amsterdam, Netherlands) · 2016Article
- Cloning and Characterization of an Endoglucanase Gene from Actinomyces sp. Korean Native Goat 40.Asian-Australasian journal of animal sciences · 2016Article
- Metagenomics: Retrospect and Prospects in High Throughput Age.Biotechnology research international · 2015Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInterest in cellulose degrading enzymes has increased in recent years due to the expansion of the ellulosic biofuel industry. The rumen is a highly adapted environment for the degradation of cellulose and a promising source of enzymes for industrial use. To identify cellulase enzymes that may be of such use we have undertaken a functional metagenomic screen to identify cellulase enzymes from the bacterial community in the rumen of a grass-hay fed dairy cow.
resultsTwenty five clones specifying cellulose activity were identified. Subcloning and sequence analysis of a subset of these hydrolase-positive clones identified 10 endoglucanase genes. Preliminary characterization of the encoded cellulases was carried out using crude extracts of each of the subclones. Zymogram analysis using carboxymethylcellulose as a substrate showed a single positive band for each subclone, confirming that only one functional cellulase gene was present in each. One cellulase gene, designated Cel14b22, was expressed at a high level in Escherichia coli and purified for further characterization. The purified recombinant enzyme showed optimal activity at pH 6.0 and 50°C. It was stable over a broad pH range, from pH 4.0 to 10.0. The activity was significantly enhanced by Mn2+ and dramatically reduced by Fe3+ or Cu2+. The enzyme hydrolyzed a wide range of beta-1,3-, and beta-1,4-linked polysaccharides, with varying activities. Activities toward microcrystalline cellulose and filter paper were relatively high, while the highest activity was toward Oat Gum.
conclusionThe present study shows that a functional metagenomic approach can be used to isolate previously uncharacterized cellulases from the rumen environment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.