ArticleBiophysical journal2012
Dynamics of protein and its hydration water: neutron scattering studies on fully deuterated GFP.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 149 citations in OpenAlex.
- Single-engineered-residue solvation perturbations regulate global protein architecture and function.Nature communications · 2026Article
- Decoupling of the onset of anharmonicity between a protein and its surface water around 200 K.eLife · 2024Article
- An experimentally representative in-silico protocol for dynamical studies of lyophilised and weakly hydrated amorphous proteins.Communications chemistry · 2024Article
- Examination of Solvent Interactions with Trp-Cage in 1,1,1,3,3,3-Hexafluoro-2-propanol-water at 298 K through MD Simulations and Intermolecular Nuclear Overhauser Effects.The journal of physical chemistry. B · 2023Article
- Spatially confined protein assembly in hierarchical mesoporous metal-organic framework.Nature communications · 2023Article
- Vibrational Analysis of Hydration-Layer Water around Ubiquitin, Unpeeled Layer by Layer: Molecular-Dynamics Perceptions.International journal of molecular sciences · 2022Article
- Tools shaping drug discovery and development.Biophysics reviews · 2022Review
- Structure and diffusive dynamics of aspartate α-decarboxylase (ADC) liganded with D-serine in aqueous solution.Physical chemistry chemical physics : PCCP · 2022Article
- The Impact of Surface Charges of Carboxylated Cellulose Nanofibrils on the Water Motions in Hydrated Films.Biomacromolecules · 2022Article
- Universal dynamical onset in water at distinct material interfaces.Chemical science · 2022Article
- Experimental mapping of short-wavelength phonons in proteins.Innovation (Cambridge (Mass.)) · 2022Article
- Seeking Solvation: Exploring the Role of Protein Hydration in Silk Gelation.Molecules (Basel, Switzerland) · 2022Article
- Water in protein hydration and ligand recognition.Journal of molecular recognition : JMR · 2019Review
- Dynamics from elastic neutron-scattering via direct measurement of the running time-integral of the van Hove distribution function.Scientific reports · 2019Article
- Water dynamics in MCF-7 breast cancer cells: a neutron scattering descriptive study.Scientific reports · 2019Article
- Dynamics Properties of Photosynthetic Microorganisms Probed by Incoherent Neutron Scattering.Biophysical journal · 2019Article
- Dynamics of a family of cyan fluorescent proteins probed by incoherent neutron scattering.Journal of the Royal Society, Interface · 2019Article
- Article
- Dynamics of water bound to crystalline cellulose.Scientific reports · 2017Article
- Investigating Structure and Dynamics of Proteins in Amorphous Phases Using Neutron Scattering.Computational and structural biotechnology journal · 2017Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a detailed analysis of the picosecond-to-nanosecond motions of green fluorescent protein (GFP) and its hydration water using neutron scattering spectroscopy and hydrogen/deuterium contrast. The analysis reveals that hydration water suppresses protein motions at lower temperatures (<~ 200 K), and facilitates protein dynamics at high temperatures. Experimental data demonstrate that the hydration water is harmonic at temperatures <~ 180-190 K and is not affected by the proteins' methyl group rotations. The dynamics of the hydration water exhibits changes at ~ 180-190 K that we ascribe to the glass transition in the hydrated protein. Our results confirm significant differences in the dynamics of protein and its hydration water at high temperatures: on the picosecond-to-nanosecond timescale, the hydration water exhibits diffusive dynamics, while the protein motions are localized to <~3 Å. The diffusion of the GFP hydration water is similar to the behavior of hydration water previously observed for other proteins. Comparison with other globular proteins (e.g., lysozyme) reveals that on the timescale of 1 ns and at equivalent hydration level, GFP dynamics (mean-square displacements and quasielastic intensity) are of much smaller amplitude. Moreover, the suppression of the protein dynamics by the hydration water at low temperatures appears to be stronger in GFP than in other globular proteins. We ascribe this observation to the barrellike structure of GFP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.