ArticleBiophysical journal2012
Peptide-lipid interactions of the stress-response peptide TisB that induces bacterial persistence.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 60 citations in OpenAlex.
- TisB enables antibiotic tolerance in Salmonella by preventing prophage induction through ATP depletion.PLoS pathogens · 2025Article
- Rational design of synthetic antimicrobial peptides based on the Escherichia coli ShoB toxin.Scientific reports · 2025Article
- Escherichia coli type I toxin TisB exclusively controls proton depolarization following antibiotic induced DNA damage.Scientific reports · 2025Article
- Type I toxin-antitoxin systems in bacteria: from regulation to biological functions.EcoSal Plus · 2024Review
- Relevance of charged and polar amino acids for functionality of membrane toxin TisB.Scientific reports · 2024Article
- TisB protein is the single molecular determinant underlying multiple downstream effects of ofloxacin inScience advances · 2024Article
- Significant influence of four highly conserved amino-acids in lipochaperon-active sHsps on the structure and functions of the Lo18 protein.Scientific reports · 2023Article
- Effects of C-Terminal Lys-Arg Residue of AapA1 Protein on Toxicity and Structural Mechanism.Toxins · 2023Article
- Mechanism of action ofFrontiers in microbiology · 2023Article
- Charged Amino Acids Contribute to ZorO Toxicity.Toxins · 2022Article
- Membranolytic Mechanism of Amphiphilic Antimicrobial β-Stranded [KL]Biomedicines · 2022Article
- Review
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- Phosphate-dependent aggregation of [KL]Scientific reports · 2020Article
- Article
- Characterization of an OrtT-like toxin of Salmonella enterica serovar Houten.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Article
- Orientation and Location of the Cyclotide Kalata B1 in Lipid Bilayers Revealed by Solid-State NMR.Biophysical journal · 2017Article
- Homo- and heteromeric interaction strengths of the synergistic antimicrobial peptides PGLa and magainin 2 in membranes.European biophysics journal : EBJ · 2016Article
- Persister formation in Staphylococcus aureus is associated with ATP depletion.Nature microbiology · 2016Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterial stress-response peptide TisB in Escherichia coli has been suggested to dissipate the transmembrane potential, such that the depletion of ATP levels induces the formation of dormant persister cells which can eventually form biofilms. We studied the structure and membrane interactions of TisB to find out whether it forms pores or other proton-selective channels. Circular dichroism revealed an amphiphilic α-helical structure when reconstituted in lipid vesicles, and oriented circular dichroism showed that the helix assumes a transmembrane alignment. The addition of TisB to dye-loaded vesicles caused leakage only at very high peptide concentration, notably with a Hill coefficient of 2, which suggests that dimers must be involved. Coarse-grained molecular dynamics simulations showed that membrane binding of monomeric TisB is rapid and spontaneous, and transmembrane insertion is energetically feasible. When TisB oligomers are assembled as transmembrane pores, these channels collapse during the simulations, but transmembrane dimers are found to be stable. Given the pattern of charges on the amphiphilic TisB helix, we postulate that antiparallel dimers could be assembled via a ladder of salt bridges. This electrostatic charge-zipper could enable protons to pass along a wire of trapped water molecules across the hydrophobic membrane.
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