ArticleMicrobial biotechnology2012
Rapid detection of total and viable Legionella pneumophila in tap water by immunomagnetic separation, double fluorescent staining and flow cytometry.
Article in Microbial biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Combination of antibody-based screening methods for the identification and quantification of Legionella pneumophila in process water.Analytical and bioanalytical chemistry · 2026Article
- Immunomagnetic separation coupled with flow cytometry for the analysis of Legionella pneumophila in aerosols.Analytical and bioanalytical chemistry · 2023Article
- Inaccuracies of the ISO 11731 Method for Environmental Validation ofMicroorganisms · 2022Article
- The impact of pipeline changes and temperature increase in a hospital historically colonised with Legionella.Scientific reports · 2021Article
- Short Ligand, Cysteine-Modified Warnericin RK Antimicrobial Peptides Favor Highly Sensitive Detection ofACS omega · 2021Article
- Enhancement of Culture of Legionella longbeachae from Respiratory Samples by Use of Immunomagnetic Separation and Antimicrobial Decontamination.Journal of clinical microbiology · 2020Article
- Persistent presence of outer membrane epitopes during short- and long-term starvation of five Legionella pneumophila strains.BMC microbiology · 2018Article
- Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review.Water research · 2017Review
- Rapid on-site monitoring of Legionella pneumophila in cooling tower water using a portable microfluidic system.Scientific reports · 2017Article
- Determination of viable legionellae in engineered water systems: Do we find what we are looking for?Water research · 2016Review
- Comparing methods of determining Legionella spp. in complex water matrices.BMC microbiology · 2015Article
- Current and emerging Legionella diagnostics for laboratory and outbreak investigations.Clinical microbiology reviews · 2015Review
- The many forms of a pleomorphic bacterial pathogen-the developmental network of Legionella pneumophila.Frontiers in microbiology · 2014Review
- Fast immunosensing technique to detect Legionella pneumophila in different natural and anthropogenic environments: comparative and collaborative trials.BMC microbiology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a rapid detection method for Legionella pneumophila (Lp) by filtration, immunomagnetic separation, double fluorescent staining, and flow cytometry (IMS-FCM method). The method requires 120 min and can discriminate 'viable' and 'membrane-damaged' cells. The recovery is over 85% of spiked Lp SG 1 cells in 1 l of tap water and detection limits are around 50 and 15 cells per litre for total and viable Lp, respectively. The method was compared using water samples from house installations in a blind study with three environmental laboratories performing the ISO 11731 plating method. In 53% of the water samples from different taps and showers significantly higher concentrations of Lp were detected by flow cytometry. No correlation to the plate culture method was found. Since also 'viable but not culturable' (VNBC) cells are detected by our method, this result was expected. The IMS-FCM method is limited by the specificity of the used antibodies; in the presented case they target Lp serogroups 1-12. This and the fact that no Lp-containing amoebae are detected may explain why in 21% of all samples higher counts were observed using the plate culture method. Though the IMS-FCM method is not yet fit to completely displace the established plating method (ISO 11731) for routine Lp monitoring, it has major advantages to plating and can quickly provide important insights into the ecology of this pathogen in water distribution systems.
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Registered trials
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