ArticleAnalytical chemistry2012
Genomic DNA extraction from cells by electroporation on an integrated microfluidic platform.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- A low-voltage alternant direct current electroporation chip for ultrafast releasing the genome DNA of Helicobacter pylori bacterium.Mikrochimica acta · 2024Article
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- CRISPR-Associated (CAS) Effectors Delivery via Microfluidic Cell-Deformation Chip.Materials (Basel, Switzerland) · 2021Review
- Continuous electrical lysis of cancer cells in a microfluidic device with passivated interdigitated electrodes.Biomicrofluidics · 2020Article
- Molecular harvesting with electroporation for tissue profiling.Scientific reports · 2019Article
- On-chip manufacturing of synthetic proteins for point-of-care therapeutics.Microsystems & nanoengineering · 2019Article
- Microfluidic reactors for advancing the MS analysis of fast biological responses.Microsystems & nanoengineering · 2019Article
- Microfluidic techniques for enhancing biofuel and biorefinery industry based on microalgae.Biotechnology for biofuels · 2019Article
- A 3D-Printed Millifluidic Platform Enabling Bacterial Preconcentration and DNA Purification for Molecular Detection of Pathogens in Blood.Micromachines · 2018Article
- Microfluidic approaches for cell-based molecular diagnosis.Biomicrofluidics · 2018Article
- Microfluidics-Based Chromosome Conformation Capture (3C) Technology for Examining Chromatin Organization with a Low Quantity of Cells.Analytical chemistry · 2018Article
- Calcein Release from Cells In Vitro via Reversible and Irreversible Electroporation.The Journal of membrane biology · 2018Article
- Towards Multiplex Molecular Diagnosis-A Review of Microfluidic Genomics Technologies.Micromachines · 2017Review
- Microfluidics for genome-wide studies involving next generation sequencing.Biomicrofluidics · 2017Review
- A mechanical cell disruption microfluidic platform based on an on-chip micropump.Biomicrofluidics · 2017Article
- High-throughput in situ cell electroporation microsystem for parallel delivery of single guide RNAs into mammalian cells.Scientific reports · 2017Article
- RNA Extraction from a Mycobacterium under Ultrahigh Electric Field Intensity in a Microfluidic Device.Analytical chemistry · 2016Article
- Article
- Individually addressable multi-chamber electroporation platform with dielectrophoresis and alternating-current-electro-osmosis assisted cell positioning.Biomicrofluidics · 2014Article
- Automated long-term monitoring of parallel microfluidic operations applying a machine vision-assisted positioning method.TheScientificWorldJournal · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
The vast majority of genetic analysis of cells involves chemical lysis for release of DNA molecules. However, chemical reagents required in the lysis interfere with downstream molecular biology and often require removal after the step. Electrical lysis based on irreversible electroporation is a promising technique to prepare samples for genetic analysis due to its purely physical nature, fast speed, and simple operation. However, there has been no experimental confirmation on whether electrical lysis extracts genomic DNA from cells in a reproducible and efficient fashion in comparison to chemical lysis, especially for eukaryotic cells that have most of the DNA enclosed in the nucleus. In this work, we construct an integrated microfluidic chip that physically traps a low number of cells, lyses the cells using electrical pulses rapidly, then purifies and concentrates genomic DNA. We demonstrate that electrical lysis offers high efficiency for DNA extraction from both eukaryotic cells (up to ∼36% for Chinese hamster ovary cells) and bacterial cells (up to ∼45% for Salmonella typhimurium) that is comparable to the widely used chemical lysis. The DNA extraction efficiency has dependence on both the electric parameters and relative amount of beads used for DNA adsorption. We envision that electroporation-based DNA extraction will find use in ultrasensitive assays that benefit from minimal dilution and simple procedures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.