ReviewMicrobes and environments2012
Are uncultivated bacteria really uncultivable?
Review in Microbes and environments, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
62 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Prevalence of unculturable bacteria in the periapical abscess: A systematic review and meta-analysis.PloS one · 2021Pooled it
- Assessing endometrial microbiota in endometriosis: culturomics and sequencing analysis of receptive-phase tissue.Current research in microbial sciences · 2026Article
- Bistability in type I toxin-antitoxin systems may lead to stress-induced persister formation.NPJ systems biology and applications · 2025Article
- Excavation of resources ofFrontiers in microbiology · 2025Article
- Natural Compounds that Modulate Circadian Rhythms.Current topics in medicinal chemistry · 2025Review
- Sequencing-guided re-estimation and promotion of cultivability for environmental bacteria.Nature communications · 2024Article
- Application of flow cytometry to analyze microbial quality of dental unit water.JADA foundational science · 2024Article
- 'Multi-omics' data integration: applications in probiotics studies.NPJ science of food · 2023Review
- The resuscitation-promoting factor (Rpf) fromAccess microbiology · 2023Article
- Reaching unreachables: Obstacles and successes of microbial cultivation and their reasons.Frontiers in microbiology · 2023Review
- Marine Bioprospecting, Biocatalysis and Process Development.Microorganisms · 2022Review
- Article
- Meta-omics approaches reveal unique small RNAs exhibited by the uncultured microorganisms dwelling deep-sea hydrothermal sediment in Guaymas Basin.Archives of microbiology · 2022Article
- Anion-type modulates the effect of salt stress on saline lake bacteria.Extremophiles : life under extreme conditions · 2022Article
- Metatranscriptomic analysis reveals active microbes and genes responded to short-term Cr(VI) stress.Ecotoxicology (London, England) · 2021Article
- Comparison of oral microbiome profile of polymers modified with silver and vanadium base nanomaterial by next-generation sequencing.Odontology · 2021Article
- Screening of Marine Bioactive Antimicrobial Compounds for Plant Pathogens.Marine drugs · 2021Review
- Functional Metagenomic Screening for Antimicrobial Resistance in the Oral Microbiome.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Multi-Replicated Enrichment Communities as a Model System in Microbial Ecology.Frontiers in microbiology · 2021Review
- Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many strategies have been used to increase the number of bacterial cells that can be grown from environmental samples but cultivation efficiency remains a challenge for microbial ecologists. The difficulty of cultivating a fraction of bacteria in environmental samples can be classified into two non-exclusive categories. Bacterial taxa with no cultivated representatives for which appropriate laboratory conditions necessary for growth are yet to be identified. The other class is cells in a non-dividing state (also known as dormant or viable but not culturable cells) that require the removal or addition of certain factors to re-initiate growth. A number of strategies, from simple to high throughput techniques, are reviewed that have been used to increase the cultivation efficiency of environmental samples. Some of the underlying mechanisms that contribute to the success of these cultivation strategies are described. Overall this review emphasizes the need of researchers to first understand the factors that are hindering cultivation to identify the best strategies to improve cultivation efficiency.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.