ArticleThe Journal of reproduction and development2013
Sexing of dog sperm by fluorescence in situ hybridization.
Article in The Journal of reproduction and development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Sperm Sexing in Selected Animals and Humans: Methods, Applications, and Future Prospects.Andrology · 2026Review
- Toll-like receptors 7 and 8 can be targeted to enrich Y-chromosome sperm in the domestic dog.Reproduction & fertility · 2026Article
- Sustainable animal production: exploring the benefits of sperm sexing technologies in addressing critical industry challenges.Frontiers in veterinary science · 2023Review
- Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs.The Journal of reproduction and development · 2022Article
- Application of the FISH Technique to Visualize Sex Chromosomes in Domestic Cat Spermatozoa.Animals : an open access journal from MDPI · 2021Article
- Clinical Cytogenetics of the Dog: A Review.Animals : an open access journal from MDPI · 2021Review
- A simple sperm-sexing method that activates TLR7/8 on X sperm for the efficient production of sexed mouse or cattle embryos.Nature protocols · 2020Article
- Advances and challenges in genetic technologies to produce single-sex litters.PLoS genetics · 2020Review
- An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm.Veterinary world · 2017Review
- Distribution of the sex chromosome during mouse spermatogenesis in testis tissue sections.The Journal of reproduction and development · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Effective preselection of sex has been accomplished in several species of livestock and also in humans using the flow cytometric sperm sorting method. A guaranteed high sorting accuracy is a key prerequisite for the widespread use of sperm sexing. The standard validation method is flow cytometric remeasurement of the DNA content of the sexed sperm. Since this method relies on the same instrument that produced the original sperm separation, it is not truly independent. Therefore, to be able to specifically produce either male or female offspring in the dog, we developed a method of direct visualization of sex chromosomes in a single sperm using fluorescence in situ hybridization (FISH) as a validation method. Denaturation of canine spermatozoa by immersion in 1 M NaOH for 4 min yielded consistent hybridization results with over 97% hybridization efficiency and a good preservation of sperm morphology. There was no significant difference between the theoretical ratio (50:50) and the observed ratio of X- and Y-chromosome-bearing spermatozoa in any of the three dogs. In addition, the mean purities of flow-sorted sex chromosomes in spermatozoa of the three dogs were 90.8% for the X chromosome fraction and 89.6% for the Y chromosome fraction. This sorting was evaluated by using the dual color FISH protocol. Therefore, our results demonstrated that the FISH protocol worked reliably for both unsorted and sexed sperm samples.
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Registered trials
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