ArticleThe International journal of oral & maxillofacial implants
Effects of coating a titanium alloy with fibronectin on the expression of osteoblast gene markers in the MC3T3 osteoprogenitor cell line.
Article in The International journal of oral & maxillofacial implants. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Engineering a Hybrid Ti6Al4V-Based System for Responsive and Consistent Osteogenesis.ACS omega · 2024Article
- The current status of stimuli-responsive nanotechnologies on orthopedic titanium implant surfaces.Journal of nanobiotechnology · 2023Review
- Aptamer-Functionalized Natural Protein-Based Polymers as Innovative Biomaterials.Pharmaceutics · 2020Review
- Commentary: A Cost-Effective Method to Enhance Adenoviral Transduction of Primary Murine Osteoblasts and Bone Marrow Stromal Cells.Frontiers in endocrinology · 2020Article
- Article
- Contributions of adhesive proteins to the cellular and bacterial response to surfaces treated with bioactive polymers: case of poly(sodium styrene sulfonate) grafted titanium surfaces.Journal of materials science. Materials in medicine · 2015Article
- The effect of five proteins on stem cells used for osteoblast differentiation and proliferation: a current review of the literature.Cellular and molecular life sciences : CMLS · 2014Review
- Structure-activity relationship of human bone sialoprotein peptides.European journal of oral sciences · 2013Article
- Heat and radiofrequency plasma glow discharge pretreatment of a titanium alloy promote bone formation and osseointegration.Journal of cellular biochemistry · 2013Article
- Heat and radiofrequency plasma glow discharge pretreatment of a titanium alloy: evidence [corrected] for enhanced osteoinductive properties.Journal of cellular biochemistry · 2013Article
- Heat or radiofrequency plasma glow discharge treatment of a titanium alloy stimulates osteoblast gene expression in the MC3T3 osteoprogenitor cell line.Journal of periodontal & implant science · 2012Article
- Impact of surface topography and coating on osteogenesis and bacterial attachment on titanium implants.Journal of tissue engineeringArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeA number of environmental and patient-related factors contribute to implant failure. A significant fraction of these failures can be attributed to limited osseointegration resulting from poor bone healing responses. The overall goal of this study was to determine whether surface treatment of a titanium-aluminum-vanadium alloy (Ti-6Al-4V) implant material in combination with a biomimetic protein coating could promote the differentiation of attached osteoblastic cells. The specific aims of the study were to investigate whether osteoprogenitor cells cultured on a rigorously cleaned implant specimen showed a normal pattern of differentiation and whether preadsorbed fibronectin accelerated or enhanced osteoblast differentiation. MATERIALS AND
methodsTi-6Al-4V disks were rigorously cleaned, passivated in nitric acid, and dry heat- sterilized; some of the disks were then coated with 1 nmol/L fibronectin. MC3T3 osteoprogenitor cells were then cultured on the pretreated disks for several weeks. Quantitative real-time polymerase chain reaction was performed to measure changes over time in the mRNA levels of osteoblast genes.
resultsFibronectin increased the peak expression of all analyzed osteoblast gene markers. "Early" genes that normally mark the proliferative phase (0 to 10 days) of osteoblastic development showed peak expression within the first 10 days after cell attachment to the titanium alloy. In contrast, "late" genes that normally mark the differentiation (10 to 20 days) and mineralization (20 to 36 days) phases of osteoblastogenesis achieved peak expression only after approximately 3 to 4 weeks of culture.
conclusionsOsteoprogenitors cultured on a rigorously cleaned Ti-6Al-4V alloy were found to demonstrate a normal pattern of osteoblast differentiation. Preadsorbed fibronectin was observed to stimulate osteoblast differentiation during the mineralization phase of osteoblastogenesis.
Indexed as
Identifiers
23057020PMC3810407W205829464What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.