ArticlePloS one2012
Optical biomarkers of serous and mucinous human ovarian tumor assessed with nonlinear optics microscopies.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- Metabolic profiling of steatotic liver disease by fluorescence lifetime imaging microscopy.Communications medicine · 2026Article
- Metabolic reprogramming enhances oxidative stress resistance in differentiating cardiomyocytes.Scientific reports · 2026Article
- Multimodal imaging of metabolic activities for distinguishing subtypes of breast cancer.Biomedical optics express · 2023Article
- Accurate evaluation of the treatment effects of immunotherapy on subcutaneous ovarian cancer in mice with nonlinear optical imaging and algorithmic analysis.Biomedical optics express · 2022Article
- Multiscale anisotropy analysis of second-harmonic generation collagen imaging of mouse skin.Journal of biomedical optics · 2021Article
- Article
- Canine mammary cancer diagnosis from quantitative properties of nonlinear optical images.Biomedical optics express · 2020Article
- Bimodal magnetic resonance and optical imaging of extracellular matrix remodelling by orthotopic ovarian tumours.British journal of cancer · 2020Article
- Label-free multiphoton imaging to assess neoadjuvant therapy responses in breast carcinoma.International journal of biological sciences · 2020Article
- Second and third harmonic generation microscopy visualizes key structural components in fresh unprocessed healthy human breast tissue.Journal of biophotonics · 2019Article
- Characterization of Pancreatic Cancer Tissue Using Multiphoton Excitation Fluorescence and Polarization-Sensitive Harmonic Generation Microscopy.Frontiers in oncology · 2019Article
- Tensor regularized total variation for denoising of third harmonic generation images of brain tumors.Journal of biophotonics · 2019Article
- A quantitative framework for the analysis of multimodal optical microscopy images.Quantitative imaging in medicine and surgery · 2017Article
- Epithelial Ovarian Cancer Diagnosis of Second-Harmonic Generation Images: A Semiautomatic Collagen Fibers Quantification Protocol.Cancer informatics · 2017Article
- Third harmonic generation imaging for fast, label-free pathology of human brain tumors.Biomedical optics express · 2016Article
- Multispectral fluorescence imaging of human ovarian and fallopian tube tissue for early-stage cancer detection.Journal of biomedical optics · 2016Article
- A Novel Intravital Imaging Window for Longitudinal Microscopy of the Mouse Ovary.Scientific reports · 2015Article
- Applications of second-harmonic generation imaging microscopy in ovarian and breast cancer.Perspectives in medicinal chemistry · 2015Article
- Neurosurgical confocal endomicroscopy: A review of contrast agents, confocal systems, and future imaging modalities.Surgical neurology international · 2014Review
- Nonlinear optical microscopy signal processing strategies in cancer.Cancer informatics · 2014Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNonlinear optical (NLO) microscopy techniques have potential to improve the early detection of epithelial ovarian cancer. In this study we showed that multimodal NLO microscopies, including two-photon excitation fluorescence (TPEF), second-harmonic generation (SHG), third-harmonic generation (THG) and fluorescence lifetime imaging microscopy (FLIM) can detect morphological and metabolic changes associated with ovarian cancer progression. METHODOLOGY/PRINCIPAL
findingsWe obtained strong TPEF + SHG + THG signals from fixed samples stained with Hematoxylin & Eosin (H&E) and robust FLIM signal from fixed unstained samples. Particularly, we imaged 34 ovarian biopsies from different patients (median age, 49 years) including 5 normal ovarian tissue, 18 serous tumors and 11 mucinous tumors with the multimodal NLO platform developed in our laboratory. We have been able to distinguish adenomas, borderline, and adenocarcinomas specimens. Using a complete set of scoring methods we found significant differences in the content, distribution and organization of collagen fibrils in the stroma as well as in the morphology and fluorescence lifetime from epithelial ovarian cells. CONCLUSIONS/SIGNIFICANCE: NLO microscopes provide complementary information about tissue microstructure, showing distinctive patterns for serous and mucinous ovarian tumors. The results provide a basis to interpret future NLO images of ovarian tissue and lay the foundation for future in vivo optical evaluation of premature ovarian lesions.
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