ArticlePloS one2012
Identification of global alteration of translational regulation in glioma in vivo.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- SelK promotes glioblastoma cell proliferation by inhibiting β-TrCP1 mediated ubiquitin-dependent degradation of CDK4.Journal of experimental & clinical cancer research : CR · 2024Article
- Distinct hypoxia-induced translational profiles of embryonic and adult-derived macrophages.iScience · 2023Article
- The presence of TIM-3 positive cells in WHO grade III and IV astrocytic gliomas correlates with isocitrate dehydrogenase mutation status.Brain pathology (Zurich, Switzerland) · 2021Article
- Article
- Raloxifene prevents stress granule dissolution, impairs translational control and promotes cell death during hypoxia in glioblastoma cells.Cell death & disease · 2020Article
- Relevance of Translation Initiation in Diffuse Glioma Biology and its Therapeutic Potential.Cells · 2019Review
- Polysome Profiling of a Human Glioblastoma Reveals Intratumoral Heterogeneity.International journal of molecular sciences · 2019Article
- Phosphatidylinositol-3 kinase-dependent translational regulation of Id1 involves the PPM1G phosphatase.Oncogene · 2016Article
- Corticosteroids compromise survival in glioblastoma.Brain : a journal of neurology · 2016Article
- Molecular portraits: the evolution of the concept of transcriptome-based cancer signatures.Briefings in bioinformatics · 2015Review
- Translational compensation of genomic instability in neuroblastoma.Scientific reports · 2015Article
- TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos.Journal of visualized experiments : JoVE · 2015Article
- Ribosome profiling reveals a cell-type-specific translational landscape in brain tumors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Most human non-GCIMP glioblastoma subtypes evolve from a common proneural-like precursor glioma.Cancer cell · 2014Article
- In vivo radiation response of proneural glioma characterized by protective p53 transcriptional program and proneural-mesenchymal shift.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Modifying chemotherapy response by targeted inhibition of eukaryotic initiation factor 4A.Blood cancer journal · 2013Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Post-transcriptional regulation of gene expression contributes to the protein output of a cell, however, methods for measuring translational regulation in complex in vivo systems are lacking. Here, we describe a sensitive method for measuring translational regulation in defined cell populations from heterogeneous tissue in vivo. We adapted the translating ribosome affinity purification (TRAP) methodology to measure the relative occupancy of individual mRNA transcripts in translating ribosomes in the Olig2-positive tumor cell population in a genetically engineered mouse model (GEM) of glioma. Global measurement of paired ribosome-bound and total cellular mRNA populations from tumor cells in vivo identified a broad distribution of relative ribosome occupancies amongst mRNA species that was highly reproducible across biological samples. Comparison of the translation state of glioma cells to non-transformed oligodendrocyte progenitor cells in normal brain identified global alteration of translation in tumor, and specifically of genes involved in cell division and synthetic metabolism. Furthermore, investigation of alteration in steady state translational efficiencies upon loss of PTEN, one of the most frequently mutated and deleted tumor suppressors in glioma, identified differential translation of proteins involved in cellular respiration, canonically regulated by PI3K/Akt signaling, and cellular glycosylation profiles, deregulation of which is known to be associated with tumor progression. Application of the translation efficiency profiling method described here to other biological contexts and conditions would extend our knowledge of the scope and impact of this important mode of gene regulation in complex in vivo systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.