ArticlePloS one2012
Protective antioxidant and antiapoptotic effects of ZnCl2 in rat pancreatic islets cultured in low and high glucose concentrations.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
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- Pig meniscus single-cell sequencing reveals highly active red zone chondrocyte populations involved in stemness maintenance and vascularization development.Journal of Zhejiang University. Science. B · 2025Article
- Pancreas-Liver-Adipose Axis: Target of Environmental Cadmium Exposure Linked to Metabolic Diseases.Toxics · 2023Review
- Zinc restores functionality in porcine prepubertal Sertoli cells exposed to subtoxic cadmium concentrationFrontiers in endocrinology · 2023Article
- Bioabsorbable metal zinc differentially affects mitochondria in vascular endothelial and smooth muscle cells.Biomaterials and biosystems · 2021Article
- Emerging Roles of Metallothioneins in Beta Cell Pathophysiology: Beyond and Above Metal Homeostasis and Antioxidant Response.Biology · 2021Review
- Toll-Like Receptor 4 and Inflammatory Micro-Environment of Pancreatic Islets in Type-2 Diabetes Mellitus: A Therapeutic Perspective.Diabetes, metabolic syndrome and obesity : targets and therapy · 2020Review
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- WTC rat has unique characteristics such as resistant to streptozotocin.Biochemistry and biophysics reports · 2016Article
- Zinc and diabetes mellitus: understanding molecular mechanisms and clinical implications.Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2015Review
- Can Tea Extracts Exert a Protective Effect Against Diabetes by Reducing Oxidative Stress and Decreasing Glucotoxicity in Pancreatic β-Cells?Diabetes & metabolism journal · 2015Article
- Mitochondrial and ER-targeted eCALWY probes reveal high levels of free Zn2+.ACS chemical biology · 2014Article
- Article
- The islet estrogen receptor-α is induced by hyperglycemia and protects against oxidative stress-induced insulin-deficient diabetes.PloS one · 2014Article
- Progesterone and cilostazol protect mice pancreatic islets from oxidative stress induced by hydrogen peroxide.Iranian journal of pharmaceutical research : IJPR · 2014Article
- Zinc supplementation protects against cadmium accumulation and cytotoxicity in Madin-Darby bovine kidney cells.PloS one · 2014Article
- Long-term culture of human pancreatic islets reveals reduced metal ion pathways in their gene signature.Cell transplantationArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aim/hypothesisRat pancreatic islet cell apoptosis is minimal after prolonged culture in 10 mmol/l glucose (G10), largely increased in 5 mmol/l glucose (G5) and moderately increased in 30 mmol/l glucose (G30). This glucose-dependent asymmetric V-shaped profile is preceded by parallel changes in the mRNA levels of oxidative stress-response genes like Metallothionein 1a (Mt1a). In this study, we tested the effect of ZnCl(2), a potent inducer of Mt1a, on apoptosis, mitochondrial oxidative stress and alterations of glucose-induced insulin secretion (GSIS) induced by prolonged exposure to low and high vs. intermediate glucose concentrations.
methodsMale Wistar rat islets were cultured in RPMI medium. Islet gene mRNA levels were measured by RTq-PCR. Apoptosis was quantified by measuring islet cytosolic histone-associated DNA fragments and the percentage of TUNEL-positive β-cells. Mitochondrial thiol oxidation was measured in rat islet cell clusters expressing "redox sensitive GFP" targeted to the mitochondria (mt-roGFP1). Insulin secretion was measured by RIA.
resultsAs observed for Mt1a mRNA levels, β-cell apoptosis and loss of GSIS, culture in either G5 or G30 vs. G10 significantly increased mt-roGFP1 oxidation. While TPEN decreased Mt1a/2a mRNA induction by G5, addition of 50-100 µM ZnCl(2) to the culture medium strongly increased Mt1a/2a mRNA and protein levels, reduced early mt-roGFP oxidation and significantly decreased late β-cell apoptosis after prolonged culture in G5 or G30 vs. G10. It did not, however, prevent the loss of GSIS under these culture conditions.
conclusionZnCl(2) reduces mitochondrial oxidative stress and improves rat β-cell survival during culture in the presence of low and high vs. intermediate glucose concentrations without improving their acute GSIS.
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