ArticlePloS one2012
Bimodal activation of different neuron classes with the spectrally red-shifted channelrhodopsin chimera C1V1 in Caenorhabditis elegans.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- RIM and RIM-Binding Protein Localize Synaptic CaV2 Channels to Differentially Regulate Transmission in Neuronal Circuits.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Glial KCNQ KNeuron · 2024Article
- Multicolor two-photon light-patterning microscope exploiting the spatio-temporal properties of a fiber bundle.Biomedical optics express · 2024Article
- Highly sensitive visual restoration and protection via ectopic expression of chimeric rhodopsin in mice.iScience · 2023Article
- Cellular and subcellular optogenetic approaches towards neuroprotection and vision restoration.Progress in retinal and eye research · 2023Review
- Towards translational optogenetics.Nature biomedical engineering · 2023Review
- Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.Frontiers in chemistry · 2022Review
- Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Genetic Methods for Cellular Manipulation in C. elegans.Methods in molecular biology (Clifton, N.J.) · 2022Article
- BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons.Nature communications · 2021Article
- Synapsin Is Required for Dense Core Vesicle Capture and cAMP-Dependent Neuropeptide Release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Theoretical optimization of high-frequency optogenetic spiking of red-shifted very fast-Chrimson-expressing neurons.Neurophotonics · 2019Article
- Investigating the feasibility of channelrhodopsin variants for nanoscale optogenetics.Neurophotonics · 2019Article
- Strong pH-Dependent Near-Infrared Fluorescence in a Microbial Rhodopsin Reconstituted with a Red-Shifting Retinal Analogue.The journal of physical chemistry letters · 2018Article
- Endophilin A and B Join Forces With Clathrin to Mediate Synaptic Vesicle Recycling inFrontiers in molecular neuroscience · 2018Article
- Rhodopsin optogenetic toolbox v2.0 for light-sensitive excitation and inhibition in Caenorhabditis elegans.PloS one · 2018Article
- Voltage imaging with genetically encoded indicators.Current opinion in chemical biology · 2017Review
- Optogenetic Approaches to Drug Discovery in Neuroscience and Beyond.Trends in biotechnology · 2017Review
- Activity of theeLife · 2016Article
- Understanding Synaptogenesis and Functional Connectome in C. elegans by Imaging Technology.Frontiers in synaptic neuroscience · 2016Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The C. elegans nervous system is particularly well suited for optogenetic analyses of circuit function: Essentially all connections have been mapped, and light can be directed at the neuron of interest in the freely moving, transparent animals, while behavior is observed. Thus, different nodes of a neuronal network can be probed for their role in controlling a particular behavior, using different optogenetic tools for photo-activation or -inhibition, which respond to different colors of light. As neurons may act in concert or in opposing ways to affect a behavior, one would further like to excite these neurons concomitantly, yet independent of each other. In addition to the blue-light activated Channelrhodopsin-2 (ChR2), spectrally red-shifted ChR variants have been explored recently. Here, we establish the green-light activated ChR chimera C1V1 (from Chlamydomonas and Volvox ChR1's) for use in C. elegans. We surveyed a number of red-shifted ChRs, and found that C1V1-ET/ET (E122T; E162T) works most reliable in C. elegans, with 540-580 nm excitation, which leaves ChR2 silent. However, as C1V1-ET/ET is very light sensitive, it still becomes activated when ChR2 is stimulated, even at 400 nm. Thus, we generated a highly efficient blue ChR2, the H134R; T159C double mutant (ChR2-HR/TC). Both proteins can be used in the same animal, in different neurons, to independently control each cell type with light, enabling a further level of complexity in circuit analyses.
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