ArticlePloS one2012
GREB1 functions as a growth promoter and is modulated by IL6/STAT3 in breast cancer.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
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- Prognostic values of four Notch receptor mRNA expression in gastric cancer.Scientific reports · 2016Article
- Distinct prognostic values of four-Notch-receptor mRNA expression in ovarian cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Predictive features of ligand-specific signaling through the estrogen receptor.Molecular systems biology · 2016Article
- Prognostic values of Notch receptors in breast cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Prognostic values of aldehyde dehydrogenase 1 isoenzymes in ovarian cancer.OncoTargets and therapy · 2016Article
- The prognostic roles of ALDH1 isoenzymes in gastric cancer.OncoTargets and therapy · 2016Article
- Distinct prognostic values of ALDH1 isoenzymes in breast cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
- Distinct prognostic values and potential drug targets of ALDH1 isoenzymes in non-small-cell lung cancer.Drug design, development and therapy · 2015Article
- mRNA profiling reveals determinants of trastuzumab efficiency in HER2-positive breast cancer.PloS one · 2015Article
- A functional interplay between ZNF217 and estrogen receptor alpha exists in luminal breast cancers.Molecular oncology · 2014Article
- Identification of the glucosyltransferase that converts hydroxymethyluracil to base J in the trypanosomatid genome.The Journal of biological chemistry · 2014Article
- Retraction: GREB1 functions as a growth promoter and is modulated by IL6/STAT3 in breast cancer.PloS one · 2014Article
- Activation of the IL-6/JAK/STAT3 signaling pathway in human middle ear cholesteatoma epithelium.International journal of clinical and experimental pathology · 2014Article
- Computational identification of novel biochemical systems involved in oxidation, glycosylation and other complex modifications of bases in DNA.Nucleic acids research · 2013Article
Corrections and comments
- Retraction · 2014-07-15Concerns/Issues about Referencing/Attributions · Investigation by Company/Institution · Investigation by Third Party · Lack of Approval from Company/Institution · Lack of Approval from Third Party ·
- Retracted
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundGrowth Regulation by Estrogen in Breast cancer (GREB1) was an estrogen receptor (ER) target gene, and GREB1 expression inversely correlated with HER2 status, possibly as a surrogate marker for ER status and a predictor for tamoxifen resistance in breast cancer patients. In the present study, we examine the function and regulation of GREB1 in breast cancer, with the goal to develop GREB1 as a biomarker in breast cancer with de novo and acquired tamoxifen resistance.
methodsWe overexpressed GREB1 using adenovirus containing the full length GREB1 cDNA (Ad-GREB1) in breast cancer cell lines. The soft agar assay was used as a measure of anchorage independent growth. The effects of GREB1 on cell proliferation in MCF-7 cells transduced with Ad-GREB1 were also measured by the me olic activity using AlamarBlue assay. We tested whether there was interaction between STAT3 and ER, which could repress GREB1 expression by immunoprecipitation assay. The effects of IL-6/JAK/STAT3 cascade activation on estrogen-induced GREB1 promoter activity were determined by luciferase assay and those on gene expression were measured by real time reverse transcription polymerase chain reaction (qRT-PCR).
resultsWe found that the ability of breast cancer cells to grow in soft agar is enhanced following GREB1 transfection. In MCF-7 cells transduced with Ad-GREB1 or transfected with siRNA GREB1, the metabolic activity was increased or completely abolished, suggesting that GREB1 may function as a growth promoter in breast cancer. E2 treatment increased GREB1 promoter luciferase activity. IL-6 inhibited E2-induced GREB1 transcription activity and GREB1 mRNA expression. Constitutively expressing active STAT3 construct (STAT3-C) dramatically decreased GREB1 transcription.
conclusionsThese data indicate that overexpression of GREB1 promotes cell proliferation and increases the clonogenic ability in breast cancer cells. Moreover, Il6/STAT3 modulates estrogen-induced GREB1 transcriptional activity in breast cancer cells.
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