ArticlePloS one2012
Transient scrotal hyperthermia induces lipid droplet accumulation and reveals a different ADFP expression pattern between the testes and liver in mice.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- Metabolic hubs in reproduction: The regulatory network of lipid droplets in gamete and embryo physiology (Review).International journal of molecular medicine · 2026Review
- Fetal Exposure to Endocrine Disrupting-Bisphenol A (BPA) Alters Testicular Fatty Acid Metabolism in the Adult Offspring: Relevance to Sperm Maturation and Quality.International journal of molecular sciences · 2023Article
- A susceptibility locus rs7099208 is associated with non-obstructive azoospermia via reduction in the expression of FAM160B1.Journal of biomedical research · 2015Article
- Mild hyperthermia enhances transport of liposomal gemcitabine and improves in vivo therapeutic response.Advanced healthcare materials · 2015Article
- Article
- Lack of testicular seipin causes teratozoospermia syndrome in men.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- Transient mild hyperthermia induces E-selectin mediated localization of mesoporous silicon vectors in solid tumors.PloS one · 2014Article
- Lipid droplet metabolism.Current opinion in clinical nutrition and metabolic care · 2013Review
- Reversibility of Spermatogonial Stem Cell Injury After Single Acute Scrotal Hyperthermia in Mouse and Rat Models: A Systematic Review.Reproductive medicine and biologyReview
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn most mammals, the testes provide a stable environment for spermatogenesis, which depends on a lower temperature than the core body temperature. It has been reported that mild testicular heating safely and reversibly suppresses spermatogenesis, and is under consideration for its potential application as a male contraceptive. Previously, we focused on the molecular mechanism of germ cell apoptosis and anti-apoptotic factors induced by heat treatment in humans and mice. However, the recovery process remains under investigation.
resultsIn this study, we found that lipid droplets in mouse testes are dramatically increased after a brief period of scrotal hyperthermia, and gradually dissipate following temperature normalization. Analysis of the human testis proteome revealed nine proteins associated with lipid droplets. Two of them, ADFP (also known as ADRP and PLIN2) and TIP47 (also known as PLIN3) may participate in acute lipid droplet formation in mammalian testes. We show that Adfp expression is upregulated after scrotal heat treatment in mice. Surprisingly, we find Adfp lacking its 5'-UTR is observed in Adfp(Δ1/Δ1) mouse testes, but is not detectable in liver.
conclusionsThese results reveal testis Adfp transcriptional regulation is tissue-specific, and is associated with lipid droplet accumulation induced by heat. The results also indicate that the testes could retain functional proteins through testes-specific transcriptional regulation.
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