ArticleGenetics and molecular biology2012
Genetic approaches for studying transgene inheritance and genetic recombination in three successive generations of transformed tobacco.
Article in Genetics and molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Article
- ThePlant direct · 2022Article
- Stability of Transgene Inheritance in Progeny of Field-Grown Pear Trees over a 7-Year Period.Plants (Basel, Switzerland) · 2022Article
- The Drought-Mediated Soybean GmNAC085 Functions as a Positive Regulator of Plant Response to Salinity.International journal of molecular sciences · 2021Article
- Ectopic expression of GmHP08 enhances resistance of transgenic Arabidopsis toward drought stress.Plant cell reports · 2021Article
- Gene Flow Risks From Transgenic Herbicide-Tolerant Crops to Their Wild Relatives Can Be Mitigated by Utilizing Alien Chromosomes.Frontiers in plant science · 2021Article
- Heterologous Expression of a Soybean GenePlants (Basel, Switzerland) · 2020Article
- CRISPR-Cas9-Mediated Mutagenesis of the Rubisco Small Subunit Family inFrontiers in genome editing · 2020Article
- The Soybean GmNAC019 Transcription Factor Mediates Drought Tolerance inInternational journal of molecular sciences · 2019Article
- Ectopic Expression ofBiomolecules · 2019Article
- High-Density Genetic Linkage Maps Provide Novel Insights Into ZW/ZZ Sex Determination System and Growth Performance in Mud Crab (Frontiers in genetics · 2019Article
- Inheritance and effectiveness of two transgenes determining PVY resistance in progeny from crossing independently transformed tobacco lines.Journal of applied genetics · 2017Article
- Genetically transformed tobacco plants expressing synthetic EPSPS gene confer tolerance against glyphosate herbicide.Physiology and molecular biology of plants : an international journal of functional plant biology · 2017Article
- Mutation scanning in a single and a stacked genetically modified (GM) event by real-time PCR and high resolution melting (HRM) analysis.International journal of molecular sciences · 2014Article
- Randomly detected genetically modified (GM) maize (Zea mays L.) near a transport route revealed a fragile 45S rDNA phenotype.PloS one · 2013Article
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2 authors.
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Abstract
Transgene integration into plant genomes is a complex process accompanied by molecular rearrangements. Classic methods that are normally used to study transgenic population genetics are generally inadequate for assessing such integration. Two major characteristics of transgenic populations are that a transgenic genome may harbor many copies of the transgene and that molecular rearrangements can create an unstable transgenic locus. In this work, we examined the segregation of T1, T2 and T3 transgenic tobacco progenies. Since transfer DNA (T-DNA) contains the NptII selectable marker gene that confers resistance to kanamycin, we used this characteristic in developing a method to estimate the number of functional inserts integrated into the genome. This approach was based on calculation of the theoretical segregation ratios in successive generations. Mendelian ratios of 3:1, 15:1 and 63:1 were confirmed for five transformation events whereas six transformation events yielded non-segregating progenies, a finding that raised questions about causal factors. A second approach based on a maximum likelihood method was performed to estimate recombination frequencies between linked inserts. Recombination estimates varied among transformation events and over generations. Some transgenic loci were unstable and evolved continuously to segregate independently in the T3 generation. Recombination and amplification of the transgene and filler DNA yielded additional transformed genotypes.
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