ArticleJournal of biochemistry2013
Accurate determination of tissue steroid hormones, precursors and conjugates in adult male rat.
Article in Journal of biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 40 citations in OpenAlex.
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- Design and Validation of a Sensitive Multisteroid LC-MS/MS Assay for the Routine Clinical Use: One-Step Sample Preparation with Phospholipid Removal and Comparison to Immunoassays.International journal of molecular sciences · 2022Article
- Simultaneous Quantification of Steroid Hormones Using hrLC-MS in Endocrine Tissues of Male Rats and Human Samples.Metabolites · 2022Article
- Markers of Local and Systemic Estrogen Metabolism in Endometriosis.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- Inhibition of Estrogen Sulfotransferase (Journal of the American Society of Nephrology : JASN · 2020Article
- Enzymatic Synthesis of Anabolic Steroid Glycosides by Glucosyltransferase fromJournal of microbiology and biotechnology · 2020Article
- Analysis of Corticosterone and Testosterone Synthesis in Rat Salivary Gland Homogenates.Frontiers in endocrinology · 2019Article
- Bioconversion of Corticosterone into Corticosterone-Glucoside by Glucosyltransferase.Molecules (Basel, Switzerland) · 2018Article
- Adrenal steroidogenesis disruption caused by HDL/cholesterol suppression in diethylstilbestrol-treated adult male rat.Endocrine · 2016Article
- Select steroid hormone glucuronide metabolites can cause toll-like receptor 4 activation and enhanced pain.Brain, behavior, and immunity · 2015Article
- Article
- Quantification of vitamin D and 25-hydroxyvitamin D in soft tissues by liquid chromatography-tandem mass spectrometry.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The actual levels of steroid hormones in organs are vital for endocrine, reproductive and neuronal health and disorders. We developed an accurate method to determine the levels of steroid hormones and steroid conjugates in various organs by an efficient preparation using a solid-phase-extraction cartridge. Each steroid was identified by the precursor ion spectra using liquid chromatography-electrospray ionization time-of-flight mass spectrometry, and the respective steroids were quantitatively analysed in the selected reaction monitoring mode by liquid chromatograph-mass spectrometry/mass spectrometry (LC-MS/MS). The data showed that significant levels of testosterone, corticosterone and precursors of both hormones were detected in all organs except liver. The glucuronide conjugates of steroid hormones and the precursors were detected in all organs except liver, but sulfate conjugates of these steroids were observed only in the target organs of the hormones and kidney. Interestingly, these steroids and the conjugates were not observed in the liver except pregnenolone. In conclusion, an accurate determination of tissue steroids was developed using LC-MS analysis. Biosynthesis of steroid hormones from the precursors was estimated even in the target organs, and the delivery of these steroid conjugates was also suggested via the circulation without any significant hepatic participation.
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Registered trials
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