ArticleThe Plant cell2012
Characterization of a NADH-dependent glutamate dehydrogenase mutant of Arabidopsis demonstrates the key role of this enzyme in root carbon and nitrogen metabolism.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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69 citing papers in PubMed, 168 citations in OpenAlex.
- Integrative Physiological-Biochemical and Multi-Omics Analyses Reveal Regulatory Mechanisms Governing Growth, Selenium Accumulation, and Nutrient Metabolism in Selenium Biologically Enhanced Quinoa (Chenopodium quinoa Willd.).Journal of agricultural and food chemistry · 2026Article
- Synergistic effect of naphthalene acetic acid and salicylic acid on the growth and tolerance mechanism of cucumber under salt stress.Scientific reports · 2026Article
- Interaction of 4-Aminobutyrate (GABA) with the Tricarboxylic Acid Cycle in Plants Under Salinity Stress.Plants (Basel, Switzerland) · 2026Review
- Nitrogen form regulates ginsenoside biosynthesis inFrontiers in plant science · 2026Article
- Transcriptomic insights into gibberellin-3-mediated regulation of bud dormancy and metabolic transition in pitaya (Hylocereus polyrhizus).Plant cell reports · 2025Article
- Joint multi-omics analysis reveals the response mechanism in rapeseed (Brassica Rapa L.) under low nitrogen stress.Functional & integrative genomics · 2025Article
- NAD(H) and NADP(H) in plants and mammals.Molecular plant · 2025Review
- Two types of covering crops are beneficial to improve the nitrogen metabolism of Citrus roots.BMC plant biology · 2025Article
- Genetic suppressor ofFrontiers in plant science · 2025Article
- Glutamate dehydrogenase from Pantoea ananatis: A new bacterial enzyme with dual coenzyme specificity.PloS one · 2025Article
- Natural variation in the adjustment of primary metabolism determines ammonium tolerance in the model grass Brachypodium distachyon.Journal of experimental botany · 2024Article
- Gaining or cutting SLAC: the evolution of plant guard cell signalling pathways.The New phytologist · 2024Article
- ArabidopsisPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Plants (Basel, Switzerland) · 2023Article
- The diversity of substrates for plant respiration and how to optimize their use.Plant physiology · 2023Review
- Transcriptome Analysis of the Effects of Grafting Interstocks on Apple Rootstocks and Scions.International journal of molecular sciences · 2023Article
- CsGDH2.1 negatively regulates theanine accumulation in late-spring tea plants (Horticulture research · 2023Article
- Nitrogen transport and assimilation in tea plant (Frontiers in plant science · 2023Review
- Low nitrogen stress-induced transcriptome changes revealed the molecular response and tolerance characteristics in maintaining the C/N balance of sugar beet (Frontiers in plant science · 2023Article
- Multiomic Approaches Reveal Hormonal Modulation and Nitrogen Uptake and Assimilation in the Initial Growth of Maize Inoculated withPlants (Basel, Switzerland) · 2022Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of NADH-dependent glutamate dehydrogenase (GDH) was investigated by studying the physiological impact of a complete lack of enzyme activity in an Arabidopsis thaliana plant deficient in three genes encoding the enzyme. This study was conducted following the discovery that a third GDH gene is expressed in the mitochondria of the root companion cells, where all three active GDH enzyme proteins were shown to be present. A gdh1-2-3 triple mutant was constructed and exhibited major differences from the wild type in gene transcription and metabolite concentrations, and these differences appeared to originate in the roots. By placing the gdh triple mutant under continuous darkness for several days and comparing it to the wild type, the evidence strongly suggested that the main physiological function of NADH-GDH is to provide 2-oxoglutarate for the tricarboxylic acid cycle. The differences in key metabolites of the tricarboxylic acid cycle in the triple mutant versus the wild type indicated that, through metabolic processes operating mainly in roots, there was a strong impact on amino acid accumulation, in particular alanine, γ-aminobutyrate, and aspartate in both roots and leaves. These results are discussed in relation to the possible signaling and physiological functions of the enzyme at the interface of carbon and nitrogen metabolism.
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