ArticleMolecular biology reports2013
Characterization of the open reading frame 7a from Bombyx mori nucleopolyhedrovirus.
Article in Molecular biology reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed, 0 citations in OpenAlex.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The open reading frame 7a of the Bombyx mori nucleopolyhedrovirus (BmNPV orf7a, Bm7a) encodes a predicted polypeptide consisting of 53 amino acid residues. While this polypeptide's homologues were found in certain baculoviruses, its function has not yet been studied. A protein structural assay revealed that the polypeptide expressed from this gene contained a predicted signal peptide at its N-terminus. To investigate the expression of Bm7a, 3'rapid amplification of cDNA ends was used to detect its transcript. A series of recombinant viruses were also constructed to analyze the cellular localization of the Bm7a products. The results indicated that the Bm7a gene is co-transcribed with bv/odv-e26 and orf9. The function of BM7a was studied by using fused enhanced green fluorescence protein (eGFP) as a visual marker to trace the localization of the protein during infection. Fluorescence was observed in the cellular membrane of the infected cells by confocal microscopy. Western blot analysis using an eGFP-antibody also showed that the BM7a-eGFP fusion protein was present in the budded virions (BVs). These data implied that BM7a is a component of the BV. In this work, we identified the function of the Bm7a gene and suggested that it may play a role in BV packaging.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.