ArticleCytotechnology2013
A novel medium for the development of in vitro cell culture system from Penaeus monodon.
Article in Cytotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 42 citations in OpenAlex.
- A primary hepatopancreatic cell platform enables cellular dissection of glucose metabolism and endocrine-metabolic regulation inFrontiers in endocrinology · 2026Article
- Transcriptional and Translational Analysis of the Expression of CHH, MIH, and GIH in PmLyO-Sf9 Cell Line Towards Developing a Novel In Vitro Model for Shrimp Endocrinology.Marine biotechnology (New York, N.Y.) · 2025Article
- Baculovirus and Plasmid Vector-Mediated Gene Transfer inBio-protocol · 2025Article
- Experimental Infection Models and Their Usefulness for White Spot Syndrome Virus (WSSV) Research in Shrimp.Viruses · 2024Review
- In vitro propagation of infectious hypodermal hematopoietic necrosis virus (Penaeus stylirostris penstyldensovirus) in PmLyO-Sf9 cells.Archives of virology · 2023Article
- An optimization of supplements and physical factors for growth of hemocytes culture from Penaeus vannamei (White shrimp) in selective medium.Molecular biology reports · 2022Article
- Status in molluscan cell line development in last one decade (2010-2020): impediments and way forward.Cytotechnology · 2022Review
- A Novel Approach of Transducing Recombinant Baculovirus into Primary Lymphoid Cells of Penaeus monodon for Developing Continuous Cell Line.Marine biotechnology (New York, N.Y.) · 2021Article
- Impaired telomerase activity hinders proliferation and in vitro transformation of Penaeus monodon lymphoid cells.Cytotechnology · 2016Article
- Transgene expression in Penaeus monodon cells: evaluation of recombinant baculoviral vectors with shrimp specific hybrid promoters.Cytotechnology · 2016Article
- Development of primary cell cultures using hemocytes and phagocytic tissue cells of Locusta migratoria: an application for locust immunity studies.In vitro cellular & developmental biology. Animal · 2016Article
- Multifactorial interaction of growth factors on Penaeus monodon lymphoid cells and the impact of IGFs in DNA synthesis and metabolic activity in vitro.Cytotechnology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lack of a valid shrimp cell line has been hampering the progress of research on shrimp viruses. One of the reasons identified was the absence of an appropriate medium which would satisfy the requirements of the cells in vitro. We report the first attempt to formulate an exclusive shrimp cell culture medium (SCCM) based on the haemolymph components of Penaeus monodon prepared in isosmotic seawater having 27 ‰ salinity. The SCCM is composed of 22 amino acids, 4 sugars, 6 vitamins, cholesterol, FBS, phenol red, three antibiotics, potassium dihydrogen phosphate and di-sodium hydrogen phosphate at pH 6.8-7.2. Osmolality was adjusted to 720 ± 10 mOsm kg(-1) and temperature of incubation was 25 ºC. The most appropriate composition was finally selected based on the extent of attachment of cells and their proliferation by visual observation. Metabolic activity of cultured cells was measured by MTT assay and compared with that in L-15 (2×), modified L-15 and Grace's insect medium, and found better performance in SCCM especially for lymphoid cells with 107 % increase in activity and 85 ± 9 days of longevity. The cells from ovary and lymphoid organs were passaged twice using the newly designed shrimp cell dissociation "cocktail".
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.