ArticlePflugers Archiv : European journal of physiology2012
Transient receptor potential canonical channel 1 impacts on mechanosignaling during cell migration.
Article in Pflugers Archiv : European journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Piezo1-induced durotaxis of pancreatic stellate cells depends on TRPC1 and TRPV4 channels.Journal of cell science · 2025Article
- Tension at the gate: sensing mechanical forces at the blood-brain barrier in health and disease.Journal of neuroinflammation · 2024Review
- Piezo1-induced durotaxis of pancreatic stellate cells depends on TRPC1 and TRPV4 channels.bioRxiv : the preprint server for biology · 2024Article
- The TRPC1 Channel Forms a PI3K/CaM Complex and Regulates Pancreatic Ductal Adenocarcinoma Cell Proliferation in a CaInternational journal of molecular sciences · 2022Article
- TRPV4 and TRPC1 channels mediate the response to tensile strain in mouse Müller cells.Cell calcium · 2022Article
- Mechanosensitive ion channels in cell migration.Cells & development · 2021Review
- RNA-seq analysis reveals TRPC genes to impact an unexpected number of metabolic and regulatory pathways.Scientific reports · 2020Article
- It takes more than two to tango: mechanosignaling of the endothelial surface.Pflugers Archiv : European journal of physiology · 2020Review
- Functional Expression of TRP Ion Channels in Endometrial Stromal Cells of Endometriosis Patients.International journal of molecular sciences · 2018Article
- The function of TRP channels in neutrophil granulocytes.Pflugers Archiv : European journal of physiology · 2018Review
- The Role of TRP Channels in the Metastatic Cascade.Pharmaceuticals (Basel, Switzerland) · 2018Review
- Mechanical Transduction and the Dark Energy of Biology.Biophysical journal · 2018Review
- TRPC6 channels modulate the response of pancreatic stellate cells to hypoxia.Pflugers Archiv : European journal of physiology · 2017Article
- Ion channels in control of pancreatic stellate cell migration.Oncotarget · 2017Article
- Role of TRPC1 channels in pressure-mediated activation of murine pancreatic stellate cells.European biophysics journal : EBJ · 2016Article
- TRPV4 participates in the establishment of trailing adhesions and directional persistence of migrating cells.Pflugers Archiv : European journal of physiology · 2015Article
- TRP channels and STIM/ORAI proteins: sensors and effectors of cancer and stroma cell migration.British journal of pharmacology · 2014Review
- Ion channels and transporters in tumour cell migration and invasion.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell migration is crucial for many important physiological and pathophysiological processes ranging from embryogenesis to tumor metastasis. It requires the coordination of mechanical forces generated in different regions of the migrating cell. It has been proposed that stretch-activated, Ca(2+)-permeable channels are involved in mechanosignaling during cell migration. To date, the molecular identity of these channels is only poorly defined. Here, we investigated the contribution of TRPC1 channels to mechanosignaling during cell migration. We used primary cultures of synovial fibroblasts from TRPC1(-/-) mice and the wild-type littermates or Madin-Darby canine kidney (MDCK-F) cells with increased or decreased TRPC1 expression. TRPC1(-/-) fibroblasts have the same migratory phenotype as siTRPC1 MDCK-F cells, with a largely increased projected cell area and impaired directionality. Measurements of the intracellular Ca(2+) concentration ([Ca(2+)](i)) were combined with time-lapse video microscopic cell migration experiments. Cells were seeded on elastic silicone membranes. Uniaxial stretch elicits a graded elevation of the [Ca(2+)](i) in TRPC1-expressing cells. In contrast, TRPC1(-/-) fibroblasts or siTRPC1 MDCK-F cells do not react to 0.4 %, and the response to 4 % stretch is attenuated. Similarly, siTRPC1 MDCK-F cells do not alter their direction of migration upon mechanical stimulation, which contrasts the behavior of TRPC1-overexpressing cells which turn into the direction of stretch. Impaired mechanosignaling in siTRPC1 MDCK-F cells leads to accelerated lamellipodial protrusions. Finally, artificially decreasing membrane tension with the detergent deoxycholate impairs the migration of TRPC1-overexpressing cells, but not of siTRPC1 cells. Taken together, our findings indicate that TRPC1 channels are linked to mechanosignaling during cell migration.
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