ArticleBiology of reproduction2012
Microbial products alter the expression of membrane-associated mucin and antimicrobial peptides in a three-dimensional human endocervical epithelial cell model.
Article in Biology of reproduction, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 61 citations in OpenAlex.
- Article
- Beyond the Barrier: Epithelial Cells as Immune Sentinels in the Female Genital Tract.American journal of reproductive immunology (New York, N.Y. : 1989) · 2026Review
- The female reproductive tract microbiotas, inflammation, and gynecological conditions: mechanisms, therapeutic advances, and future perspectives.Frontiers in microbiology · 2026Review
- Human Cervix Chip: A Preclinical Model for Studying the Role of the Cervical Mucosa and Microbiome in Female Reproductive Health.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Mucus production, host-microbiome interactions, hormone sensitivity, and innate immune responses modeled in human cervix chips.Nature communications · 2024Article
- Article
- Ovarian sex steroid and epithelial control of immune responses in the uterus and oviduct: human and animal models†.Biology of reproduction · 2024Article
- Commensal Lactobacilli Metabolically Contribute to Cervical Epithelial Homeostasis in a Species-Specific Manner.mSphere · 2023Article
- Endometrial Microbiota and Immune Tolerance in Pregnancy.International journal of molecular sciences · 2023Review
- Interactions between microbiota and cervical epithelial, immune, and mucus barrier.Frontiers in cellular and infection microbiology · 2023Review
- In vitro and ex vivo models for evaluating vaginal drug delivery systems.Advanced drug delivery reviews · 2022Review
- DoesCancers · 2022Review
- Immunometabolic and potential tumor-promoting changes in 3D cervical cell models infected with bacterial vaginosis-associated bacteria.Communications biology · 2022Article
- Mucus secretions from a conditionally reprogrammed primary endocervical cell culture.F&S science · 2022Article
- The Interplay Between Cervicovaginal Microbial Dysbiosis and Cervicovaginal Immunity.Frontiers in immunology · 2022Review
- The female reproductive tract microbiotas, inflammation, and gynecological conditions.Frontiers in reproductive health · 2022Review
- "Iron triangle" of regulating the uterine microecology: Endometrial microbiota, immunity and endometrium.Frontiers in immunology · 2022Review
- Bacterial vaginosis and health-associated bacteria modulate the immunometabolic landscape in 3D model of human cervix.NPJ biofilms and microbiomes · 2021Article
- The Role of Antimicrobial Peptides in Preterm Birth.International journal of molecular sciences · 2021Review
- Veillonellaceae family members uniquely alter the cervical metabolic microenvironment in a human three-dimensional epithelial model.NPJ biofilms and microbiomes · 2021Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Our understanding of the mechanisms that regulate tissue-specific mucosal defense can be limited by the lack of appropriate human in vitro models. The endocervix lies between the microbe-rich vaginal cavity and the relatively sterile endometrium and is a major portal of entry for Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, human immunodeficiency virus (HIV), and herpes simplex virus (HSV) infection in women. The endocervix is lined with a simple epithelium, and these cells produce mucus, which plays a key role in immune defense and reproduction. Here we describe the development of a human three-dimensional endocervical epithelial cell model generated by rotating wall vessel bioreactor technology. The model is composed of cellular aggregates that recapitulate major structural and barrier properties essential for the function and protection of the endocervix, including junctional complexes, microvilli, innate immune receptors, antimicrobial peptides, and mucins, the major structural component of mucus. Using this model, we also report, for the first time, that the membrane-associated mucin genes MUC1, MUC4, and MUC16 are differentially regulated in these aggregates by different bacterial and viral products. Differential induction of antimicrobial peptides was also observed with these products. Together these data define unique and flexible innate endocervical immune signatures that follow exposure to microbial products and that likely play a critical role in the outcome of pathogen challenge at this site.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.