ArticleParasitology research2013
Protective immunity induced by a DNA vaccine encoding Eimeria tenella rhomboid against homologous challenge.
Article in Parasitology research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Effects of prebiotic (lactoferrin) and diclazuril on broiler chickens experimentally infected withFrontiers in veterinary science · 2024Article
- Vaccines against chicken coccidiosis with particular reference to previous decade: progress, challenges, and opportunities.Parasitology research · 2022Review
- A multiepitope vaccine encoding four Eimeria epitopes with PLGA nanospheres: a novel vaccine candidate against coccidiosis in laying chickens.Veterinary research · 2022Article
- Evaluation of immunoprotective effects of recombinant proteins and DNA vaccines derived from Eimeria tenella surface antigen 6 and 15 in vivo.Parasitology research · 2022Article
- Protective efficacy induced byFrontiers in veterinary science · 2022Article
- Evaluation of immunoprotective effects of recombinant protein and DNA vaccine based on Eimeria tenella surface antigen 16 and 22 in vivo.Parasitology research · 2021Article
- Article
- Rhomboid protein 2 of Eimeria maxima provided partial protection against infection by homologous species.Veterinary research · 2021Article
- A review of Eimeria antigen identification for the development of novel anticoccidial vaccines.Parasitology research · 2019Review
- Article
- Effect of Dual Infection withFrontiers in veterinary science · 2017Article
- DNA vaccination of poultry: The current status in 2015.Vaccine · 2016Review
- Protective efficacy in chickens of recombinant plasmid pET32a(+)-ADF-3-1E of Eimeria acervulina.Parasitology research · 2014Article
- Effect of dinitolmide intercalated into Montmorillonite on E. tenella infection in chickens.Parasitology research · 2014Article
- Eimeria maxima: efficacy of recombinant Mycobacterium bovis BCG expressing apical membrane antigen1 against homologous infection.Parasitology research · 2013Article
- Partial protective of chickens against Eimeria tenella challenge with recombinant EtMIC-1 antigen.Parasitology research · 2013Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhomboid protein in Apicomplexa was associated with the process of host cell invasion. To evaluate the potential of the protein in eliciting protective immunity against challenge, a DNA vaccine pVAX1-Rho encoding Eimeria tenella rhomboid was constructed. Recombinant protein was expressed in Hela cells and verified by indirect immunofluorescence and western blotting analysis. In vivo experiments, 1-week-old chickens were randomly divided into three groups. Experimental group of chickens were immunized with DNA vaccines while control group of chickens were injected with pVAX1 plasmid alone or sterile water. Two weeks following the booster dose, all chickens were inoculated orally with 5 × 10(4) sporulated oocysts of E. tenella. The host immunity and protective efficacy of this vaccine against E. tenella challenge in broilers were evaluated. Results showed that specific antibody, the levels of interleukin-2 (IL-2), interferon-γ (IFN-γ), and the percentages of CD4(+) and CD8(+) T lymphocyte cells were significantly increased in the pVAX1-Rho group. Challenge experiments demonstrated that pVAX1-Rho vaccination could reduce oocyst excretion, decrease cecal lesion, increase bodyweight gains and provide chickens with oocysts decrease ratio around 75.8 %. These results suggest that the pVAX1-Rho was able to induce humoral and cellular responses and generate protective immunity against E. tenella infection.
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