ArticleJournal of clinical microbiology2012
Advanced yellow fever virus genome detection in point-of-care facilities and reference laboratories.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 117 papers.
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Who cites it
117 citing papers in PubMed.
- Strengthening diagnostic capacity for viral hemorrhagic fevers in Forest Guinea: advances in Lassa fever case detection.Npj viruses · 2026Article
- Analytical and Clinical Evaluation of the STANDARD M10 Arbovirus Panel for Dengue Detection, Serotyping, and Multiplex Arboviral Screening in the Americas.Diagnostics (Basel, Switzerland) · 2026Article
- Observational
- Aminoglycosides induce immune activation but is insufficient for systemic viral control: a double-blind randomized-controlled clinical trial.Communications medicine · 2026Article
- Evolution and spillover dynamics of yellow fever at the forest-urban interface in Brazil.Nature microbiology · 2026Article
- Entomo-virological investigation during the epizootic outbreak of sylvatic yellow fever in Rio Grande do Sul, Brazil, between 2021 and 2022Memorias do Instituto Oswaldo Cruz · 2026Article
- Technical validation and implementation of a multiplex real-time PCR for differential diagnostic detection of Zika virus, yellow fever virus, dengue virus and chikungunya virus (ZYDC-PCR).BMC infectious diseases · 2026Article
- Development and validation of a sample-to-result real time multiplex RT-PCR test for human African trypanosomiasis and arboviral febrile illnesses in the Democratic Republic of the Congo.BMC infectious diseases · 2026Observational
- Yellow fever virus resurgence in Sao Paulo State, Brazil, 2024-2025.Revista do Instituto de Medicina Tropical de Sao Paulo · 2026Article
- Integrated surveillance of arboviruses in febrile patients from the Brazilian Amazon reveals complex co-circulation dynamics and hidden viral diversity.Revista da Sociedade Brasileira de Medicina Tropical · 2026Article
- Surveillance Service of Yellow Fever in Non-Human Primates in the Federal District, Brazil, 2008-2022.Revista da Sociedade Brasileira de Medicina Tropical · 2026Article
- Limited IgM seroreactivity to arboviruses in free-ranging black-tufted marmosets (Frontiers in veterinary science · 2026Article
- Reemergence of yellow fever virus in forest and periurban settings in Brazil.Communications medicine · 2025Article
- Yellow Fever Virus in Aedes albopictus Mosquitoes from Urban Green Area, São Paulo State, Brazil.Emerging infectious diseases · 2025Article
- Nationwide surveillance detects yellow fever and chikungunya viruses in multiple Aedes mosquito species in Nigeria.Parasites & vectors · 2025Article
- Article
- Uncovering Hidden Transmission: Active Surveillance Reveals Cryptic Circulation of Yellow Fever Virus in Urban Marmosets in Belo Horizonte, Brazil, 2024.Pathogens (Basel, Switzerland) · 2025Article
- Assessing Urban Yellow Fever Transmission Risk:Viruses · 2025Article
- Human Herpesvirus 1 Associated with Epizootics in Belo Horizonte, Minas Gerais, Brazil.Viruses · 2025Article
- Re-emergence of Yellow Fever Virus in Brazil: Evidence from Forest and peri-urban Settings.medRxiv : the preprint server for health sciences · 2025Article
57 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reported methods for the detection of the yellow fever viral genome are beset by limitations in sensitivity, specificity, strain detection spectra, and suitability to laboratories with simple infrastructure in areas of endemicity. We describe the development of two different approaches affording sensitive and specific detection of the yellow fever genome: a real-time reverse transcription-quantitative PCR (RT-qPCR) and an isothermal protocol employing the same primer-probe set but based on helicase-dependent amplification technology (RT-tHDA). Both assays were evaluated using yellow fever cell culture supernatants as well as spiked and clinical samples. We demonstrate reliable detection by both assays of different strains of yellow fever virus with improved sensitivity and specificity. The RT-qPCR assay is a powerful tool for reference or diagnostic laboratories with real-time PCR capability, while the isothermal RT-tHDA assay represents a useful alternative to earlier amplification techniques for the molecular diagnosis of yellow fever by field or point-of-care laboratories.
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