ArticleMolecular biology of the cell2012
Establishing epithelial glandular polarity: interlinked roles for ARF6, Rac1, and the matrix microenvironment.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 24 citations in OpenAlex.
- The calcium pump PMCA4b promotes epithelial cell polarization and lumen formation.Communications biology · 2025Article
- The scaffold protein IQGAP1 promotes reorientation of epithelial cell polarity at the two-cell stage for cystogenesis.Genes to cells : devoted to molecular & cellular mechanisms · 2024Article
- Arf6 as a therapeutic target: Structure, mechanism, and inhibitors.Acta pharmaceutica Sinica. B · 2023Review
- Rab22a regulates the establishment of epithelial polarity.Small GTPases · 2021Article
- Tunable Hybrid Matrices Drive Epithelial Morphogenesis and YAP Translocation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Epithelial polarization in 3D matrix requires DDR1 signaling to regulate actomyosin contractility.Life science alliance · 2019Article
- Mesenchymal-epithelial transition in development and reprogramming.Nature cell biology · 2019Review
- Aberrant endocytosis leads to the loss of normal mitotic spindle orientation during epithelial glandular morphogenesis.The Journal of biological chemistry · 2018Article
- Laminins in Epithelial Cell Polarization: Old Questions in Search of New Answers.Cold Spring Harbor perspectives in biology · 2017Review
- Article
- Rab14 specifies the apical membrane through Arf6-mediated regulation of lipid domains and Cdc42.Scientific reports · 2016Article
- Regulation of podocalyxin trafficking by Rab small GTPases in epithelial cells.Small GTPases · 2016Article
- Human eccrine sweat gland cells reconstitute polarized spheroids when subcutaneously implanted with Matrigel in nude mice.Journal of molecular histology · 2016Article
- Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells.Nature communications · 2016Article
- Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures.The Journal of cell biology · 2016Article
- Rab35 GTPase couples cell division with initiation of epithelial apico-basal polarity and lumen opening.Nature communications · 2016Article
- Phosphoinositide 3-kinase p110δ promotes lumen formation through the enhancement of apico-basal polarity and basal membrane organization.Nature communications · 2015Article
- The Role of ARF6 in Biliary Atresia.PloS one · 2015Article
- FE65 interacts with ADP-ribosylation factor 6 to promote neurite outgrowth.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- ARF6-regulated endocytosis of growth factor receptors links cadherin-based adhesion to canonical Wnt signaling in epithelia.Molecular and cellular biology · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Epithelial cysts comprise the structural units of the glandular epithelium. Although glandular inversion in epithelial tumors is thought to be a potential mechanism for the establishment of metastatic disease, little is known about the morphogenic cues and signaling pathways that govern glandular polarity and organization. Using organotypic cultures of Madin-Darby canine kidney cells in reconstituted basement membrane, we show that cellular depletion of the small GTP-binding protein ARF6 promotes the formation of inverted cysts, wherein the apical cell membrane faces the cyst exterior, and the basal domain faces the central lumen, while individual cell polarity is maintained. These cysts are also defective in interactions with laminin at the cyst-matrix interface. This inversion of glandular orientation is accompanied by Rac1 inactivation during early cystogenesis, and temporal activation of Rac1 is sufficient to recover the normal cyst phenotype. In an unnatural collagen I microenvironment, ARF6-depleted, inverted epithelial cysts exhibit some loss of cell polarity, a marked increase in Rho activation and Rac1 inactivation, and striking rearrangement of the surrounding collagen I matrix. These studies demonstrate the importance of ARF6 as a critical determinant of glandular orientation and the matrix environment in dictating structural organization of epithelial cysts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.