ArticleG3 (Bethesda, Md.)2012
Light-inducible system for tunable protein expression in Neurospora crassa.
Article in G3 (Bethesda, Md.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 55 citations in OpenAlex.
- How fungi see the world: fungal photoreceptors and their role in the regulation of fungal biology.Microbiology and molecular biology reviews : MMBR · 2025Review
- Tuning fungal promoters for the expression of eukaryotic proteins.World journal of microbiology & biotechnology · 2024Review
- Use of the Saccharomycopsis schoenii MET17 promoter for regulated heterologous gene expression.Current genetics · 2024Article
- Methylxanthines Modulate Circadian Period Length Independently of the Action of Phosphodiesterase.Microbiology spectrum · 2023Article
- Identification and characterization of sugar-regulated promoters in Chaetomium thermophilum.BMC biotechnology · 2023Article
- Article
- Introducing Protein Homeostasis and Glycogen Synthesis as Two Targets of Blue Light Radiation inJournal of lasers in medical sciences · 2022Article
- Shannon entropy as a metric for conditional gene expression in Neurospora crassa.G3 (Bethesda, Md.) · 2021Article
- Article
- Article
- Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fungi.Applied microbiology and biotechnology · 2018Review
- Light-regulated promoters for tunable, temporal, and affordable control of fungal gene expression.Applied microbiology and biotechnology · 2018Review
- Up-Frameshift Protein UPF1 RegulatesGenetics · 2017Article
- Establishment of Neurospora crassa as a host for heterologous protein production using a human antibody fragment as a model product.Microbial cell factories · 2017Article
- Codon usage is an important determinant of gene expression levels largely through its effects on transcription.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Modulation of Circadian Gene Expression and Metabolic Compensation by the RCO-1 Corepressor of Neurospora crassa.Genetics · 2016Article
- A Tool Set for the Genome-Wide Analysis of Neurospora crassa by RT-PCR.G3 (Bethesda, Md.) · 2015Article
- Metabolic Impacts of Using Nitrogen and Copper-Regulated Promoters to Regulate Gene Expression in Neurospora crassa.G3 (Bethesda, Md.) · 2015Article
- Suppressing the Neurospora crassa circadian clock while maintaining light responsiveness in continuous stirred tank reactors.Scientific reports · 2015Article
- How to train your microbe: methods for dynamically characterizing gene networks.Current opinion in microbiology · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Filamentous fungi are important model systems for understanding eukaryotic cellular processes, including the study of protein expression. A salient feature of fungi is the ability of the protein-processing machinery to perform all of the extensive posttranslational modifications needed in the complex world of eukaryotic organisms, making them great hosts for production of eukaryotic proteins. In the model organism Neurospora crassa, several regulatable promoters have been used for heterologous gene expression but all suffer from leaky expression absent stimuli or an inability to induce protein expression at levels greater than those seen in vivo. To increase and better control in vivo protein expression in Neurospora, we have harnessed the light-induced vvd promoter. vvd promoter-driven mRNA expression is dependent upon light, shows a graded response, and is rapidly shut off when returned to the dark. The vvd promoter is a highly tunable and regulatable system, which could be a useful instrument for those interested in efficient and controllable gene expression.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.