ArticleScientific reports2012
Multiplex epitope mapping using bacterial surface display reveals both linear and conformational epitopes.
Article in Scientific reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Proteomic Applications in Vaccine Development.Advances in experimental medicine and biology · 2026Review
- Rapid Mapping of Protein Binding Sites and Conformational Epitopes by Coupling Yeast Surface Display to Chemical Labeling and Deep Sequencing.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Mapping of Antibody Structural Epitopes Using Bacterial Cell Surface Display of Folded Domains.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Fully synthetic platform to rapidly generate tetravalent bispecific nanobody-based immunoglobulins.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A SH3_5 Cell Anchoring Domain for Non-recombinant Surface Display on Lactic Acid Bacteria.Frontiers in bioengineering and biotechnology · 2020Article
- Expression and Characterization of Human Vascular Endothelial Growth Factor Produced in SiHa Cells Transduced with Adenoviral Vector.The protein journal · 2019Article
- Surface Display of Small Affinity Proteins on Synechocystis sp. Strain PCC 6803 Mediated by Fusion to the Major Type IV Pilin PilA1.Journal of bacteriology · 2018Article
- Prediction of antibody structural epitopes via random peptide library screening and next generation sequencing.Journal of immunological methods · 2017Article
- Staphylococcus carnosus: from starter culture to protein engineering platform.Applied microbiology and biotechnology · 2017Review
- Antibody Engineering for Pursuing a Healthier Future.Frontiers in microbiology · 2017Review
- Functional characterization of a monoclonal antibody epitope using a lambda phage display-deep sequencing platform.Scientific reports · 2016Article
- Stratification of responders towards eculizumab using a structural epitope mapping strategy.Scientific reports · 2016Article
- Article
- Peptide-based vaccinology: experimental and computational approaches to target hypervariable viruses through the fine characterization of protective epitopes recognized by monoclonal antibodies and the identification of T-cell-activating peptides.Clinical & developmental immunology · 2013Review
- Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As antibody-based diagnosis and therapy grow at an increased pace, there is a need for methods which rapidly and accurately determine antibody-antigen interactions. Here, we report a method for the multiplex determination of antibody epitopes using bacterial cell-surface display. A protein-fragment library with 10(7) cell clones, covering 60 clinically-relevant protein targets, was created and characterized with massively parallel sequencing. Using this multi-target fragment library we determined simultaneously epitopes of commercial monoclonal and polyclonal antibodies targeting PSMA, EGFR, and VEGF. Off-target binding was observed for one of the antibodies, which demonstrates the methods ability to reveal cross-reactivity. We exemplify the detection of structural epitopes by mapping the therapeutic antibody Avastin. Based on our findings we suggest this method to be suitable for mapping linear and structural epitopes of monoclonal and polyclonal antibodies in a multiplex fashion and could find applicability in serum profiling as well as other protein-protein interaction studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.