ArticlePLoS neglected tropical diseases2012
Leishmania metacyclogenesis is promoted in the absence of purines.
Article in PLoS neglected tropical diseases, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 55 citations in OpenAlex.
- Surface enolase is a virulence factor ofFrontiers in microbiology · 2026Article
- Gene deletion as a possible strategy adopted by New World Leishmania infantum to maximize geographic dispersion.PLoS pathogens · 2025Article
- Understanding the genetic complexity of Leishmania infantum in the Americas: a focus on 3'NT/NU gene deletion.Memorias do Instituto Oswaldo Cruz · 2025Review
- Identification and characterization of yeast SNF1 kinase homologs inFrontiers in molecular biosciences · 2025Article
- TORC1 is an essential regulator of nutrient-controlled proliferation and differentiation in Leishmania.EMBO reports · 2024Article
- Leishmania Ribosomal Protein (RP) paralogous genes compensate each other's expression maintaining protein native levels.PloS one · 2024Article
- Exploring Novel Drug Combinations: The Therapeutic Potential of Selanyl Derivatives for Leishmania Treatment.Molecules (Basel, Switzerland) · 2023Article
- Metabolic Pathways ofPharmaceutics · 2022Review
- Sand flies: Basic information on the vectors of leishmaniasis and their interactions with Leishmania parasites.Communications biology · 2022Review
- Stimulation of metacyclogenesis inFrontiers in cellular and infection microbiology · 2022Article
- Finding Correlations Between mRNA and Protein Levels inFrontiers in cellular and infection microbiology · 2022Review
- Nutrient sensing in Leishmania: Flagellum and cytosol.Molecular microbiology · 2021Review
- An RNA-binding protein complex regulates the purine-dependent expression of a nucleobase transporter in trypanosomes.Nucleic acids research · 2021Article
- The gene expression of Leishmania infantum chagasi inside Lutzomyia longipalpis, the main vector of visceral leishmaniasis in Brazil.Memorias do Instituto Oswaldo Cruz · 2021Article
- Purine-responsive expression of theThe Journal of biological chemistry · 2020Article
- Regulation of Translation in the Protozoan ParasiteInternational journal of molecular sciences · 2020Review
- Establishment, optimisation and quantitation of a bioluminescent murine infection model of visceral leishmaniasis for systematic vaccine screening.Scientific reports · 2020Article
- Deletion of a Single LeishIF4E-3 Allele by the CRISPR-Cas9 System Alters Cell Morphology and Infectivity ofmSphere · 2019Article
- Nutritional stress targets LeishIF4E-3 to storage granules that contain RNA and ribosome components in Leishmania.PLoS neglected tropical diseases · 2019Article
- Sequential blood meals promote Leishmania replication and reverse metacyclogenesis augmenting vector infectivity.Nature microbiology · 2018Article
Corrections and comments
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Authors and funding
14 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leishmania parasites, the causative agent of leishmaniasis, are transmitted through the bite of an infected sand fly. Leishmania parasites present two basic forms known as promastigote and amastigote which, respectively, parasitizes the vector and the mammalian hosts. Infection of the vertebrate host is dependent on the development, in the vector, of metacyclic promastigotes, however, little is known about the factors that trigger metacyclogenesis in Leishmania parasites. It has been generally stated that "stressful conditions" will lead to development of metacyclic forms, and with the exception of a few studies no detailed analysis of the molecular nature of the stress factor has been performed. Here we show that presence/absence of nucleosides, especially adenosine, controls metacyclogenesis both in vitro and in vivo. We found that addition of an adenosine-receptor antagonist to in vitro cultures of Leishmania amazonensis significantly increases metacyclogenesis, an effect that can be reversed by the presence of specific purine nucleosides or nucleobases. Furthermore, our results show that proliferation and metacyclogenesis are independently regulated and that addition of adenosine to culture medium is sufficient to recover proliferative characteristics for purified metacyclic promastigotes. More importantly, we show that metacyclogenesis was inhibited in sand flies infected with Leishmania infantum chagasi that were fed a mixture of sucrose and adenosine. Our results fill a gap in the life cycle of Leishmania parasites by demonstrating how metacyclogenesis, a key point in the propagation of the parasite to the mammalian host, can be controlled by the presence of specific purines.
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