ArticlePloS one2012
NAP1-assisted nucleosome assembly on DNA measured in real time by single-molecule magnetic tweezers.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Always on the Move: Overview on Chromatin Dynamics within Nuclear Processes.Biochemistry · 2025Review
- Nap1 and Kap114 co-chaperone H2A-H2B and facilitate targeted histone release in the nucleus.The Journal of cell biology · 2025Article
- Dynamics of nucleosomes and chromatin fibers revealed by single-molecule measurements.BMB reports · 2025Review
- Exploring TRF2-Dependent DNA Distortion Through Single-DNA Manipulation Studies.Communications biology · 2024Article
- DNA-binding proteins studied by mechanical manipulation and AFM imaging of single DNA molecules.Biophysics reports · 2022Article
- The fork protection complex recruits FACT to reorganize nucleosomes during replication.Nucleic acids research · 2022Article
- Cisplatin fastens chromatin irreversibly even at a high chloride concentration.Nucleic acids research · 2021Article
- Unsupervised selection of optimal single-molecule time series idealization criterion.Biophysical journal · 2021Article
- A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins.Molecules (Basel, Switzerland) · 2021Article
- Article
- ATRX limits the accessibility of histone H3-occupied HSV genomes during lytic infection.PLoS pathogens · 2021Article
- Nucleosome Dynamics during Transcription Elongation.ACS chemical biology · 2020Article
- DNA Sequence Is a Major Determinant of Tetrasome Dynamics.Biophysical journal · 2019Article
- The supercoiling state of DNA determines the handedness of both H3 and CENP-A nucleosomes.Nanoscale · 2017Article
- Recent insights from in vitro single-molecule studies into nucleosome structure and dynamics.Biophysical reviews · 2016Review
- Article
- Transcriptional Repressor TrmBL2 from Thermococcus kodakarensis Forms Filamentous Nucleoprotein Structures and Competes with Histones for DNA Binding in a Salt- and DNA Supercoiling-dependent Manner.The Journal of biological chemistry · 2015Article
- Histone chaperone-mediated nucleosome assembly process.PloS one · 2015Article
- Comparing the Assembly and Handedness Dynamics of (H3.3-H4)2 Tetrasomes to Canonical Tetrasomes.PloS one · 2015Article
- Copper-free click chemistry for attachment of biomolecules in magnetic tweezers.BMC biophysics · 2015Article
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Authors and funding
4 authors.
Funding
Abstract
While many proteins are involved in the assembly and (re)positioning of nucleosomes, the dynamics of protein-assisted nucleosome formation are not well understood. We study NAP1 (nucleosome assembly protein 1) assisted nucleosome formation at the single-molecule level using magnetic tweezers. This method allows to apply a well-defined stretching force and supercoiling density to a single DNA molecule, and to study in real time the change in linking number, stiffness and length of the DNA during nucleosome formation. We observe a decrease in end-to-end length when NAP1 and core histones (CH) are added to the dsDNA. We characterize the formation of complete nucleosomes by measuring the change in linking number of DNA, which is induced by the NAP1-assisted nucleosome assembly, and which does not occur for non-nucleosomal bound histones H3 and H4. By rotating the magnets, the supercoils formed upon nucleosome assembly are removed and the number of assembled nucleosomes can be counted. We find that the compaction of DNA at low force is about 56 nm per assembled nucleosome. The number of compaction steps and associated change in linking number indicate that NAP1-assisted nucleosome assembly is a two-step process.
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