ArticlePloS one2012
A Bacillus flagellar motor that can use both Na+ and K+ as a coupling ion is converted by a single mutation to use only Na+.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 63 citations in OpenAlex.
- The exceptional form and function of the giant bacteriumProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit.Nature communications · 2023Article
- Ion-Powered Rotary Motors: Where Did They Come from and Where They Are Going?International journal of molecular sciences · 2023Review
- The rapid evolution of flagellar ion selectivity in experimental populations ofScience advances · 2022Article
- Charged Lipids Influence Phase Separation in Cell-Sized Liposomes Containing Cholesterol or Ergosterol.Membranes · 2022Article
- Novel transient cytoplasmic rings stabilize assembling bacterial flagellar motors.The EMBO journal · 2022Article
- Bacterial Proprioception: Can a Bacterium Sense Its Movement?Frontiers in microbiology · 2022Review
- NaInternational journal of molecular sciences · 2021Article
- Rhizobial Chemotaxis and Motility Systems at Work in the Soil.Frontiers in plant science · 2021Review
- The Dynamic Ion Motive Force Powering the Bacterial Flagellar Motor.Frontiers in microbiology · 2021Review
- Coupling Ion Specificity of the Flagellar Stator Proteins MotA1/MotB1 ofBiomolecules · 2020Article
- MotP Subunit is Critical for Ion Selectivity and Evolution of a KBiomolecules · 2020Article
- Evolution of the Stator Elements of Rotary Prokaryote Motors.Journal of bacteriology · 2020Review
- Ancestral Sequence Reconstructions of MotB Are Proton-Motile and Require MotA for Motility.Frontiers in microbiology · 2020Article
- Dynamic exchange of two types of stator units inComputational and structural biotechnology journal · 2020Review
- Functional Regulators of Bacterial Flagella.Annual review of microbiology · 2019Review
- Essential ion binding residues for NaScientific reports · 2019Article
- Flagella-Driven Motility of Bacteria.Biomolecules · 2019Review
- The presence and absence of periplasmic rings in bacterial flagellar motors correlates with stator type.eLife · 2019Article
- Molecular dynamics simulation of bacterial flagella.Biophysical reviews · 2018Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In bacteria, the sodium ion (Na(+)) cycle plays a critical role in negotiating the challenges of an extremely alkaline and sodium-rich environment. Alkaliphilic bacteria that grow optimally at high pH values use Na(+) for solute uptake and flagellar rotation because the proton (H(+)) motive force is insufficient for use at extremely alkaline pH. Only three types of electrically driven rotary motors exist in nature: the F-type ATPase, the V-type ATPase, and the bacterial flagellar motor. Until now, only H(+) and Na(+) have been reported as coupling ions for these motors. Here, we report that the alkaliphilic bacterium Bacillus alcalophilus Vedder 1934 can grow not only under a Na(+)-rich and potassium ion (K(+))-poor condition but also under the opposite condition in an extremely alkaline environment. In this organism, swimming performance depends on concentrations of Na(+), K(+) or Rb(+). In the absence of Na(+), swimming behavior is clearly K(+)- dependent. This pattern was confirmed in swimming assays of stator-less Bacillus subtilis and Escherichia coli mutants expressing MotPS from B. alcalophilus (BA-MotPS). Furthermore, a single mutation in BA-MotS was identified that converted the naturally bi-functional BA-MotPS to stators that cannot use K(+) or Rb(+). This is the first report that describes a flagellar motor that can use K(+) and Rb(+) as coupling ions. The finding will affect the understanding of the operating principles of flagellar motors and the molecular mechanisms of ion selectivity, the field of the evolution of environmental changes and stresses, and areas of nanotechnology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.