ArticlePloS one2012
Phenylalanine hydroxylase from Legionella pneumophila is a thermostable enzyme with a major functional role in pyomelanin synthesis.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
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- Classic Pentachlorophenol Hydroxylating Phenylalanine 4-Monooxygenase from Indigenous Bacillus tropicus Strain AOA-CPS1: Cloning, Overexpression, Purification, Characterization and Structural Homology Modelling.Applied biochemistry and biotechnology · 2022Article
- Whole-genome sequencing, genome mining, metabolic reconstruction and evolution of pentachlorophenol and other xenobiotic degradation pathways in Bacillus tropicus strain AOA-CPS1.Functional & integrative genomics · 2021Article
- GapMind: Automated Annotation of Amino Acid Biosynthesis.mSystems · 2020Article
- Inhibition of Tryptophan Hydroxylases and Monoamine Oxidase-A by the Proton Pump Inhibitor, Omeprazole-Frontiers in pharmacology · 2020Article
- Gut-Brain Axis in the Early Postnatal Years of Life: A Developmental Perspective.Frontiers in integrative neuroscience · 2020Review
- The LetA/S two-component system regulates transcriptomic changes that are essential for the culturability of Legionella pneumophila in water.Scientific reports · 2018Article
- Biofilm Formation and Heat Stress Induce Pyomelanin Production in Deep-SeaFrontiers in microbiology · 2017Article
- Disruption of phenylalanine hydroxylase reduces adult lifespan and fecundity, and impairs embryonic development in parthenogenetic pea aphids.Scientific reports · 2016Article
- Stable preparations of tyrosine hydroxylase provide the solution structure of the full-length enzyme.Scientific reports · 2016Article
- Selective requirement of the shikimate pathway of Legionella pneumophila for intravacuolar growth within human macrophages but not within Acanthamoeba.Infection and immunity · 2015Article
- Article
- An update on iron acquisition by Legionella pneumophila: new pathways for siderophore uptake and ferric iron reduction.Future microbiology · 2015Review
- Secreted pyomelanin of Legionella pneumophila promotes bacterial iron uptake and growth under iron-limiting conditions.Infection and immunity · 2013Article
- Structural and thermodynamic insight into phenylalanine hydroxylase from the human pathogen Legionella pneumophila.FEBS open bio · 2013Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundLegionella pneumophila is a pathogenic bacterium that can cause Legionnaires' disease and other non-pneumonic infections in humans. This bacterium produces a pyomelanin pigment, a potential virulence factor with ferric reductase activity. In this work, we have investigated the role of phenylalanine hydroxylase from L. pneumophila (lpPAH), the product of the phhA gene, in the synthesis of the pyomelanin pigment and the growth of the bacterium in defined compositions. METHODOLOGY/PRINCIPAL
findingsComparative studies of wild-type and phhA mutant corroborate that lpPAH provides the excess tyrosine for pigment synthesis. phhA and letA (gacA) appear transcriptionally linked when bacteria were grown in buffered yeast extract medium at 37°C. phhA is expressed in L. pneumophila growing in macrophages. We also cloned and characterized lpPAH, which showed many characteristics of other PAHs studied so far, including Fe(II) requirement for activity. However, it also showed many particular properties such as dimerization, a high conformational thermal stability, with a midpoint denaturation temperature (T(m)) = 79 ± 0.5°C, a high specific activity at 37°C (10.2 ± 0.3 µmol L-Tyr/mg/min) and low affinity for the substrate (K(m) (L-Phe) = 735 ± 50 µM. CONCLUSIONS/SIGNIFICANCE: lpPAH has a major functional role in the synthesis of pyomelanin and promotes growth in low-tyrosine media. The high thermal stability of lpPAH might reflect the adaptation of the enzyme to withstand relatively high survival temperatures.
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