ArticlePloS one2012
Identification of anaphase promoting complex substrates in S. cerevisiae.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Role of Nuclear SUMO-Targeted Ubiquitin Ligase Uls2 (Slx5-Slx8) in Quality Control of Cytosolic Proteins.Biomolecules · 2026Article
- Nrm1 is a bistable switch connecting cell cycle progression to transcriptional control.EMBO reports · 2025Article
- Transient septin sumoylation steers a Fir1-Skt5 protein complex between the split septin ring.The Journal of cell biology · 2024Article
- The APC/C Activator Cdh1p Plays a Role in Mitochondrial Metabolic Remodelling in Yeast.International journal of molecular sciences · 2023Article
- Article
- Rpn4 and proteasome-mediated yeast resistance to ethanol includes regulation of autophagy.Applied microbiology and biotechnology · 2020Article
- Checkpoint Regulation of Nuclear Tos4 Defines S Phase Arrest in Fission Yeast.G3 (Bethesda, Md.) · 2020Article
- Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy.Journal of visualized experiments : JoVE · 2019Article
- A cell separation checkpoint that enforces the proper order of late cytokinetic events.The Journal of cell biology · 2019Article
- Cell-Cycle Modulation of Transcription Termination Factor Sen1.Molecular cell · 2018Article
- Identification of Genes inG3 (Bethesda, Md.) · 2017Article
- Erratum to: Controlling the response to DNA damage by the APC/C-Cdh1.Cellular and molecular life sciences : CMLS · 2016Article
- Controlling the response to DNA damage by the APC/C-Cdh1.Cellular and molecular life sciences : CMLS · 2016Review
- Degradation of Ndd1 by APC/C(Cdh1) generates a feed forward loop that times mitotic protein accumulation.Nature communications · 2015Article
- Regulation of a transcription factor network by Cdk1 coordinates late cell cycle gene expression.The EMBO journal · 2014Article
- The anaphase promoting complex regulates yeast lifespan and rDNA stability by targeting Fob1 for degradation.Genetics · 2014Article
- Cell cycle-regulated transcription: effectively using a genomics toolbox.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Tos4 mediates gene expression homeostasis through interaction with HDAC complexes independently of H3K56 acetylation.The Journal of biological chemistryArticle
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The Anaphase-Promoting Complex/Cyclosome (APC/C) is an essential ubiquitin ligase that targets numerous proteins for proteasome-mediated degradation in mitosis and G1. To gain further insight into cellular pathways controlled by APC/C(Cdh1), we developed two complementary approaches to identify additional APC/C(Cdh1) substrates in budding yeast. First, we analyzed the stabilities of proteins that were expressed at the same time in the cell cycle as known APC/C substrates. Second, we screened for proteins capable of interacting with the Cdh1 substrate-binding protein in a yeast two-hybrid system. Here we characterize five potential APC/C substrates identified using these approaches: the transcription factors Tos4 and Pdr3; the mRNA processing factor Fir1; the spindle checkpoint protein kinase Mps1; and a protein of unknown function, Ybr138C. Analysis of the degradation motifs within these proteins revealed that the carboxyl-terminal KEN box and D-boxes of Tos4 are important for its interaction with Cdh1, whereas the N-terminal domain of Ybr138C is required for its instability. Functionally, we found that a stabilized form of Mps1 delayed cell division upon mild spindle disruption, and that elevated levels of Ybr138C reduced cell fitness. Interestingly, both Tos4 and Pdr3 have been implicated in the DNA damage response, whereas Mps1 regulates the spindle assembly checkpoint. Thus, the APC/C(Cdh1)-mediated degradation of these proteins may help to coordinate re-entry into the cell cycle following environmental stresses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.