ArticlePloS one2012
A systematic investigation of differential effects of cell culture substrates on the extent of artifacts in single-molecule tracking.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Single-Molecule FRET-Tracking of InlB-Activated MET Receptors in Living Cells.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- A perspective of fluorescence microscopy for cellular structural biology with EGFR as witness.Journal of microscopy · 2023Article
- Choosing the Probe for Single-Molecule Fluorescence Microscopy.International journal of molecular sciences · 2022Review
- Fluorescence Imaging of Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor Resistance in Non-Small Cell Lung Cancer.Cancers · 2022Review
- The Cyclophilin-Dependent Calcineurin Inhibitor Voclosporin Inhibits SARS-CoV-2 Replication in Cell Culture.Transplant international : official journal of the European Society for Organ Transplantation · 2022Article
- Flow radiocytometry using droplet optofluidics.Biosensors & bioelectronics · 2021Article
- Imaging therapeutic peptide transport across intestinal barriers.RSC chemical biology · 2021Review
- Membrane dynamics are slowed for Alexa594-labeled membrane proteins due to substrate interactions.BBA advances · 2021Article
- Surface passivation of zero-mode waveguide nanostructures: benchmarking protocols and fluorescent labels.Scientific reports · 2020Article
- Solid immersion microscopy images cells under cryogenic conditions with 12 nm resolution.Communications biology · 2019Article
- Cluster Analysis of Endogenous HER2 and HER3 Receptors in SKBR3 Cells.Bio-protocol · 2018Article
- Article
- Easy and low-cost stable positioning of suspension cells during live-cell imaging.Journal of biological methods · 2017Article
- A starting point for fluorescence-based single-molecule measurements in biomolecular research.Molecules (Basel, Switzerland) · 2014Review
- Choose your label wisely: water-soluble fluorophores often interact with lipid bilayers.PloS one · 2014Article
- Hydrophobic fluorescent probes introduce artifacts into single molecule tracking experiments due to non-specific binding.PloS one · 2013Article
- Measuring EGFR separations on cells with ~10 nm resolution via fluorophore localization imaging with photobleaching.PloS one · 2013Article
- Single molecule fluorescence detection and tracking in mammalian cells: the state-of-the-art and future perspectives.International journal of molecular sciences · 2012Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Single-molecule techniques are being increasingly applied to biomedical investigation, notwithstanding the numerous challenges they pose in terms of signal-to-noise ratio issues. Non-specific binding of probes to glass substrates, in particular, can produce experimental artifacts due to spurious molecules on glass, which can be particularly deleterious in live-cell tracking experiments. In order to resolve the issue of non-specific probe binding to substrates, we performed systematic testing of a range of available surface coatings, using three different proteins, and then extended our assessment to the ability of these coatings to foster cell growth and retain non-adhesive properties. Linear PEG, a passivating agent commonly used both in immobilized-molecule single-molecule techniques and in tissue engineering, is able to both successfully repel non-specific adhesion of fluorescent probes and to foster cell growth when functionalized with appropriate adhesive peptides. Linear PEG treatment results in a significant reduction of tracking artifacts in EGFR tracking with Affibody ligands on a cell line expressing EGFR-eGFP. The findings reported herein could be beneficial to a large number of experimental situations where single-molecule or single-particle precision is required.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.