ArticlePloS one2012
Exploiting the combination of natural and genetically engineered resistance to cassava mosaic and cassava brown streak viruses impacting cassava production in Africa.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 96 citations in OpenAlex.
- RepA Enhances Plant Resistance to Citrus Chlorotic Dwarf-Associated Virus by Transcriptionally Activating ClAOS.Molecular plant pathology · 2026Article
- Unveiling the underlying complexities in breeding for disease resistance in crop plants: review.Frontiers in plant science · 2025Review
- Article
- CRISPR/Cas9: an advanced platform for root and tuber crops improvement.Frontiers in genome editing · 2023Review
- PepYLCIV and PepYLCAV resistance gene Pepy-2 encodes DFDGD-Class RNA-dependent RNA polymerase in Capsicum.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Cassava mosaic disease and its management in Southeast Asia.Plant molecular biology · 2022Review
- Modelling cassava production and pest management under biotic and abiotic constraints.Plant molecular biology · 2022Review
- Topological clustering of regulatory genes confers pathogenic tolerance to cassava brown streak virus (CBSV) in cassava.Scientific reports · 2021Article
- Efficient Genetic Transformation and Regeneration of a Farmer-Preferred Cassava Cultivar From Ghana.Frontiers in plant science · 2021Article
- Screening for Resistance in Farmer-Preferred Cassava Cultivars from Ghana to a Mixed Infection of CBSV and UCBSV.Plants (Basel, Switzerland) · 2020Article
- Incidence, severity and distribution of Cassava brown streak disease in northeastern Democratic Republic of Congo.Cogent food & agriculture · 2020Review
- RNA silencing ofBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- Engineering Disease-Resistant Cassava.Cold Spring Harbor perspectives in biology · 2019Review
- Review
- Cassava brown streak disease: historical timeline, current knowledge and future prospects.Molecular plant pathology · 2018Review
- Molecular insights into Cassava brown streak virus susceptibility and resistance by profiling of the early host response.Molecular plant pathology · 2018Article
- Novel Bioengineered Cassava Expressing an Archaeal Starch Degradation System and a Bacterial ADP-Glucose Pyrophosphorylase for Starch Self-Digestibility and Yield Increase.Frontiers in plant science · 2018Article
- QTL associated with resistance to cassava brown streak and cassava mosaic diseases in a bi-parental cross of two Tanzanian farmer varieties, Namikonga and Albert.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- Review
- A rapid virus-induced gene silencing (VIGS) method for assessing resistance and susceptibility to cassava mosaic disease.Virology journal · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) are currently two major viral diseases that severely reduce cassava production in large areas of Sub-Saharan Africa. Natural resistance has so far only been reported for CMD in cassava. CBSD is caused by two virus species, Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). A sequence of the CBSV coat protein (CP) highly conserved between the two virus species was used to demonstrate that a CBSV-CP hairpin construct sufficed to generate immunity against both viral species in the cassava model cultivar (cv. 60444). Most of the transgenic lines showed high levels of resistance under increasing viral loads using a stringent top-grafting method of inoculation. No viral replication was observed in the resistant transgenic lines and they remained free of typical CBSD root symptoms 7 month post-infection. To generate transgenic cassava lines combining resistance to both CBSD and CMD the hairpin construct was transferred to a CMD-resistant farmer-preferred Nigerian landrace TME 7 (Oko-Iyawo). An adapted protocol allowed the efficient Agrobacterium-based transformation of TME 7 and the regeneration of transgenic lines with high levels of CBSV-CP hairpin-derived small RNAs. All transgenic TME 7 lines were immune to both CBSV and UCBSV infections. Further evaluation of the transgenic TME 7 lines revealed that CBSD resistance was maintained when plants were co-inoculated with East African cassava mosaic virus (EACMV), a geminivirus causing CMD. The innovative combination of natural and engineered virus resistance in farmer-preferred landraces will be particularly important to reducing the increasing impact of cassava viral diseases in Africa.
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