ArticleThe Journal of biological chemistry2012
Permeation of calcium through purified connexin 26 hemichannels.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 93 citations in OpenAlex.
- Influence of extracellular magnesium ions on the functional state and biophysical and structural properties of connexin36 hemichannels.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- High Activity of Hemichannels Permeable to Calcium Ions Leads to ROS Generation and Reduced Cell Viability.International journal of molecular sciences · 2026Article
- The Unexplored Role of Connexin Hemichannels in Promoting Facioscapulohumeral Muscular Dystrophy Progression.International journal of molecular sciences · 2025Review
- Mechano-activated connexin hemichannels and glutathione transport protect lens fiber cells against oxidative insults.Redox biology · 2024Article
- CaProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Hemichannels contribute to mitochondrial CaFrontiers in cell and developmental biology · 2024Article
- Measurement of CaMethods in molecular biology (Clifton, N.J.) · 2024Article
- Purification, Reconstitution, and Functional Analysis of Connexin Hemichannels.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Acute activation of hemichannels by ethanol leads to CaFrontiers in cell and developmental biology · 2024Article
- SARS-CoV-2 spike protein S1 activates Cx43 hemichannels and disturbs intracellular CaBiological research · 2023Article
- Connexins in Cancer, the Possible Role of Connexin46 as a Cancer Stem Cell-Determining Protein.Biomolecules · 2023Review
- A Chemical Chaperone Restores Connexin 26 Mutant Activity.ACS pharmacology & translational science · 2023Article
- X-linked Charcot Marie Tooth mutations alter COFrontiers in cellular neuroscience · 2023Article
- Hypertensive Nephropathy: Unveiling the Possible Involvement of Hemichannels and Pannexons.International journal of molecular sciences · 2022Review
- TNF-α Plus IL-1β Induces Opposite Regulation of Cx43 Hemichannels and Gap Junctions in Mesangial Cells through a RhoA/ROCK-Dependent Pathway.International journal of molecular sciences · 2022Article
- A Quantitative Assay for CaInternational journal of molecular sciences · 2022Article
- Oxidative Stress, Inflammation and Connexin Hemichannels in Muscular Dystrophies.Biomedicines · 2022Review
- Expression of KID syndromic mutation Cx26S17F produces hyperactive hemichannels in supporting cells of the organ of Corti.Frontiers in cell and developmental biology · 2022Article
- Shared and distinct mechanisms of skeletal muscle atrophy: A narrative review.Ageing research reviews · 2021Review
- Lipid Membrane Mimetics in Functional and Structural Studies of Integral Membrane Proteins.Membranes · 2021Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundIndirect evidence suggests that connexin hemichannels are permeable to Ca(2+), but direct demonstration is lacking.
resultsCalcium moves into liposomes containing purified Cx26 in response to a concentration gradient.
conclusionCx26 hemichannels are permeable to Ca(2+). SIGNIFICANCE: Cx26 hemichannels may play a role in Ca(2+) influx into cells under conditions that lead to hemichannel activation, such as ischemic damage. Gap junction channels communicate the cytoplasms of two cells and are formed by head to head association of two hemichannels, one from each of the cells. Gap junction channels and hemichannels are permeable to ions and hydrophilic molecules of up to M(r) 1,000, including second messengers and metabolites. Intercellular Ca(2+) signaling can occur by movement of a number of second messengers, including Ca(2+), through gap junction channels, or by a paracrine pathway that involves activation of purinergic receptors in neighboring cells following ATP release through hemichannels. Understanding Ca(2+) permeation through Cx26 hemichannels is important to assess the role of gap junction channels and hemichannels in health and disease. In this context, it is possible that increased Ca(2+) influx through hemichannels under ischemic conditions contributes to cell damage. Previous studies suggest Ca(2+) permeation through hemichannels, based on indirect arguments. Here, we demonstrate for the first time hemichannel permeability to Ca(2+) by measuring Ca(2+) transport through purified Cx26 hemichannels reconstituted in liposomes. We trapped the low affinity Ca(2+)-sensitive fluorescent probe Fluo-5N into the liposomes and followed the increases in intraliposomal [Ca(2+)] in response to an imposed [Ca(2+)] gradient. We show that Ca(2+) does move through Cx26 hemichannels and that the permeability of the hemichannels to Ca(2+) is high, similar to that for Na(+). We suggest that hemichannels can be a significant pathway for Ca(2+) influx into cells under conditions such as ischemia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.