ArticleProceedings of the National Academy of Sciences of the United States of America2012
Quantitative imaging of endosome acidification and single retrovirus fusion with distinct pools of early endosomes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Fusogenic structural changes in arenavirus glycoproteins are associated with viroporin activity.PLoS pathogens · 2023Article
- pH-dependent endocytosis mechanisms for influenza A and SARS-coronavirus.Frontiers in microbiology · 2023Review
- Stimuli-responsive nanoformulations for CRISPR-Cas9 genome editing.Journal of nanobiotechnology · 2022Review
- Human LAMP1 accelerates Lassa virus fusion and potently promotes fusion pore dilation upon forcing viral fusion with non-endosomal membrane.PLoS pathogens · 2022Article
- A library of Rhodamine6G-based pH-sensitive fluorescent probes with versatileRSC chemical biology · 2022Article
- Fully Automated Computational Approach for Precisely Measuring Organelle Acidification with Optical pH Sensors.ACS applied materials & interfaces · 2022Article
- Endosomes supporting fusion mediated by vesicular stomatitis virus glycoprotein have distinctive motion and acidification.Traffic (Copenhagen, Denmark) · 2022Article
- Physicochemical tools for studying virus interactions with targeted cell membranes in a molecular and spatiotemporally resolved context.Analytical and bioanalytical chemistry · 2021Review
- Amphiphilic Histidine-Based Oligopeptides Exhibit pH-Reversible Fibril Formation.ACS macro letters · 2021Article
- Fusion and fission events regulate endosome maturation and viral escape.Scientific reports · 2021Article
- Human parvovirus B19 interacts with globoside under acidic conditions as an essential step in endocytic trafficking.PLoS pathogens · 2021Article
- CuS@PDA-FA nanocomposites: a dual stimuli-responsive DOX delivery vehicle with ultrahigh loading level for synergistic photothermal-chemotherapies on breast cancer.Journal of materials chemistry. B · 2020Article
- Article
- Single Virion Tracking Microscopy for the Study of Virus Entry Processes in Live Cells and Biomimetic Platforms.Advances in experimental medicine and biology · 2019Review
- Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube (SWCNT)-delivered MALAT1 Antisense Oligos.Journal of visualized experiments : JoVE · 2018Article
- Real-Time Dissecting the Entry and Intracellular Dynamics of Single Reovirus Particle.Frontiers in microbiology · 2018Article
- An improved labeling strategy enables automated detection of single-virus fusion and assessment of HIV-1 protease activity in single virions.The Journal of biological chemistry · 2017Article
- Single-molecule fluorescence microscopy review: shedding new light on old problems.Bioscience reports · 2017Review
- pH regulation in early endosomes and interferon-inducible transmembrane proteins control avian retrovirus fusion.The Journal of biological chemistry · 2017Article
- Recent Advances in Biosensor Development for Foodborne Virus Detection.Nanotheranostics · 2017Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Diverse enveloped viruses enter host cells through endocytosis and fuse with endosomal membranes upon encountering acidic pH. Currently, the pH dynamics in virus-carrying endosomes and the relationship between acidification and viral fusion are poorly characterized. Here, we examined the entry of avian retrovirus that requires two sequential stimuli--binding to a cognate receptor and low pH--to undergo fusion. A genetically encoded sensor incorporated into the viral membrane was used to measure the pH in virus-carrying endosomes. Acid-induced virus fusion was visualized as the release of a fluorescent viral content marker into the cytosol. The pH values in early acidic endosomes transporting the virus ranged from 5.6 to 6.5 but were relatively stable over time for a given vesicle. Analysis of viral motility and luminal pH showed that cells expressing the transmembrane isoform of the receptor (TVA950) preferentially sorted the virus into slowly trafficking, less acidic endosomes. In contrast, viruses internalized by cells expressing the GPI-anchored isoform (TVA800) were uniformly distributed between stationary and mobile compartments. We found that the lag times between acidification and fusion were significantly shorter and fusion pores were larger in dynamic endosomes than in more stationary compartments. Despite the same average pH within mobile compartments of cells expressing alternative receptor isoforms, TVA950 supported faster fusion than TVA800 receptor. Collectively, our results suggest that fusion steps downstream of the low-pH trigger are modulated by properties of intracellular compartments harboring the virus.
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