ArticleBMC microbiology2012
Rapid identification of Burkholderia mallei and Burkholderia pseudomallei by intact cell Matrix-assisted Laser Desorption/Ionisation mass spectrometric typing.
Article in BMC microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 64 citations in OpenAlex.
- Human infections caused by pathogenic Burkholderia: current clinical challenges and future perspectives.Infection · 2026Review
- The evolution of diagnostic microbiology: integrating culture-based methods and genomic advances.PeerJ · 2026Review
- Stability assessment of whole cell ELISA for Burkholderia pseudomallei antibodies detection.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- A MALDI-ToF mass spectrometry database for identification and classification of highly pathogenic bacteria.Scientific data · 2025Article
- Performance of MALDI-TOF MS, real-time PCR, antigen detection, and automated biochemical testing for the identification ofJournal of clinical microbiology · 2024Article
- Glanders Diagnosis in an Asymptomatic Mare from Brazil: Insights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing.Pathogens (Basel, Switzerland) · 2023Article
- Research on the establishment and application of protein fingerprint spectrum database ofNew microbes and new infections · 2021Article
- Determination and Identification of Antibiotic Drugs and Bacterial Strains in Biological Samples.Molecules (Basel, Switzerland) · 2020Review
- Human Melioidosis.Clinical microbiology reviews · 2020Review
- Medical Devices for Low- and Middle-Income Countries: A Review and Directions for Development.Journal of medical devices · 2020Review
- A Machine Learning-Based Raman Spectroscopic Assay for the Identification ofMolecules (Basel, Switzerland) · 2019Article
- Identification of Burkholderia pseudomallei by Use of the Vitek Mass Spectrometer.Journal of clinical microbiology · 2019Article
- Rapid antimicrobial susceptibility testing and β-lactam-induced cell morphology changes of Gram-negative biological threat pathogens by optical screening.BMC microbiology · 2018Article
- Utility of a Rapid Lateral Flow Assay To Resolve Erroneous Identification of Burkholderia pseudomallei as Burkholderia thailandensis by Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry.Journal of clinical microbiology · 2018Article
- Antibodies in Melioidosis: The Role of the Indirect Hemagglutination Assay in Evaluating Patients and Exposed Populations.The American journal of tropical medicine and hygiene · 2018Article
- Comparison of MastEuropean journal of microbiology & immunology · 2017Article
- Article
- Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for rapid identification of mold and yeast cultures of Penicillium marneffei.BMC microbiology · 2016Article
- Verification of a Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Method for Diagnostic Identification of High-Consequence Bacterial Pathogens.Journal of clinical microbiology · 2016Article
- Laboratory diagnosis of melioidosis: past, present and future.Experimental biology and medicine (Maywood, N.J.) · 2015Review
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Authors and funding
11 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBurkholderia (B.) pseudomallei and B. mallei are genetically closely related species. B. pseudomallei causes melioidosis in humans and animals, whereas B. mallei is the causative agent of glanders in equines and rarely also in humans. Both agents have been classified by the CDC as priority category B biological agents. Rapid identification is crucial, because both agents are intrinsically resistant to many antibiotics. Matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-TOF MS) has the potential of rapid and reliable identification of pathogens, but is limited by the availability of a database containing validated reference spectra. The aim of this study was to evaluate the use of MALDI-TOF MS for the rapid and reliable identification and differentiation of B. pseudomallei and B. mallei and to build up a reliable reference database for both organisms.
resultsA collection of ten B. pseudomallei and seventeen B. mallei strains was used to generate a library of reference spectra. Samples of both species could be identified by MALDI-TOF MS, if a dedicated subset of the reference spectra library was used. In comparison with samples representing B. mallei, higher genetic diversity among B. pseudomallei was reflected in the higher average Eucledian distances between the mass spectra and a broader range of identification score values obtained with commercial software for the identification of microorganisms. The type strain of B. pseudomallei (ATCC 23343) was isolated decades ago and is outstanding in the spectrum-based dendrograms probably due to massive methylations as indicated by two intensive series of mass increments of 14 Da specifically and reproducibly found in the spectra of this strain.
conclusionsHandling of pathogens under BSL 3 conditions is dangerous and cumbersome but can be minimized by inactivation of bacteria with ethanol, subsequent protein extraction under BSL 1 conditions and MALDI-TOF MS analysis being faster than nucleic amplification methods. Our spectra demonstrated a higher homogeneity in B. mallei than in B. pseudomallei isolates. As expected for closely related species, the identification process with MALDI Biotyper software (Bruker Daltonik GmbH, Bremen, Germany) requires the careful selection of spectra from reference strains. When a dedicated reference set is used and spectra of high quality are acquired, it is possible to distinguish both species unambiguously. The need for a careful curation of reference spectra databases is stressed.
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