ArticleDNA and cell biology2012
Differential regulation of the mouse and human Wnt5a alternative promoters A and B.
Article in DNA and cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed.
- Review
- Article
- Differential expression of WNT5A long and short isoforms in non-muscle-invasive bladder urothelial carcinoma.Histology and histopathology · 2024Article
- Altered binding affinity of SIX1-Q177R correlates with enhanced WNT5A and WNT pathway effector expression in Wilms tumor.Disease models & mechanisms · 2023Article
- Haspin balances the ratio of asymmetric cell division through Wnt5a and regulates cell fate decisions in mouse embryonic stem cells.Cell death discovery · 2023Article
- cRel and Wnt5a/Frizzled 5 Receptor-Mediated Inflammatory Regulation Reveal Novel Neuroprotectin D1 Targets for Neuroprotection.Cellular and molecular neurobiology · 2023Article
- The molecular mechanisms of remodeling in asthma, COPD and IPF with a special emphasis on the complex role of Wnt5A.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2023Review
- Targeted splicing therapy: new strategies for colorectal cancer.Frontiers in oncology · 2023Review
- Hematopoietic stem cell regeneration through paracrine regulation of the Wnt5a/Prox1 signaling axis.The Journal of clinical investigation · 2022Article
- The human WNT5A isoforms display similar patterns of expression but distinct and overlapping activities in normal human osteoblasts.Journal of cellular biochemistry · 2021Article
- LGK974 suppresses lipopolysaccharide-induced endotoxemia in mice by modulating the crosstalk between the Wnt/β-catenin and NF-κB pathways.Experimental & molecular medicine · 2021Article
- HIF2 Regulates Intestinal Wnt5a Expression.Frontiers in oncology · 2021Article
- WNT5a in Colorectal Cancer: Research Progress and Challenges.Cancer management and research · 2021Review
- The emerging role of Wnt5a in the promotion of a pro-inflammatory and immunosuppressive tumor microenvironment.Cancer metastasis reviews · 2020Review
- Hyperoxia Injury in the Developing Lung Is Mediated by Mesenchymal Expression of Wnt5A.American journal of respiratory and critical care medicine · 2020Article
- WNT ligands contribute to the immune response during septic shock and amplify endotoxemia-driven inflammation in mice.Blood advances · 2017Article
- Wnt5a is elevated in heart failure and affects cardiac fibroblast function.Journal of molecular medicine (Berlin, Germany) · 2017Article
- RelA/p65 inhibition prevents tendon adhesion by modulating inflammation, cell proliferation, and apoptosis.Cell death & disease · 2017Article
- Distinct roles and differential expression levels of Wnt5a mRNA isoforms in colorectal cancer cells.PloS one · 2017Article
- Microglia-induced activation of non-canonical Wnt signaling aggravates neurodegeneration in demyelinating disorders.Molecular and cellular biology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Wnt5a is an extracellular glycoprotein that activates Wnt signaling pathways, important in development and tissue homeostasis. Wnt5a expression is often misregulated during cancer progression. In this study, we analyzed the transcriptional regulation of two of the Wnt5a alternative promoters, termed A and B. Transient transfection of promoter A and B luciferase reporter constructs in to NIH3T3 and Caco-2 cells indicated that the separated promoters are both functional and that 300-450 base pair (bp) of upstream sequence is sufficient for activity. Promoter B constructs displayed distinct patterns of expression in the two cell types. The endogenous levels of promoter A-derived transcripts were found to be greater than the promoter B transcripts by four- to sixfold in fibroblast cells. Treatment of NIH3T3 cells with tumor necrosis factor (TNF)-alpha leads to an increase in both promoter A and B activities, but promoter B was more responsive. Using inhibitors of TNF-alpha effector proteins, we provide evidence that the transcription factor nuclear factor-kappaB and the MEK1/2 and p38 kinases have distinct roles in determining the activity levels of promoters, A and B. These results support the conclusion that Wnt5a promoters, A and B, are differentially regulated and provide a model for complex transcriptional regulation of Wnt5a.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.