ArticleProceedings of the National Academy of Sciences of the United States of America2012
Efficient targeted gene disruption in Xenopus embryos using engineered transcription activator-like effector nucleases (TALENs).
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 126 papers.
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Who cites it
126 citing papers in PubMed, 241 citations in OpenAlex.
- CRISPR technology in ovarian cancer research: advances from gene editing to precision diagnosis and therapy.Translational cancer research · 2026Review
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- Systematic review:Frontiers in medicine · 2025Review
- Intestinal remodeling during Xenopus metamorphosis as a model for studying thyroid hormone signaling and adult organogenesis.Molecular and cellular endocrinology · 2024Article
- Simplifying Genotyping of Mutants from Genome Editing with a Parallel qPCR-Based iGenotype Index.Cells · 2024Article
- Cell cycle activation in thyroid hormone-induced apoptosis and stem cell development duringFrontiers in endocrinology · 2023Review
- Steroid-receptor coactivator complexes in thyroid hormone-regulation of Xenopus metamorphosis.Vitamins and hormones · 2023Article
- Tissue-Targeted CRISPR-Cas9-Mediated Genome Editing of Multiple Homeologs in FCold Spring Harbor protocols · 2022Article
- Gene editing monkeys: Retrospect and outlook.Frontiers in cell and developmental biology · 2022Review
- The application of genome editing technology in fish.Marine life science & technology · 2021Review
- Life Without Thyroid Hormone Receptor.Endocrinology · 2021Review
- The development of adult intestinal stem cells: Insights from studies on thyroid hormone-dependent anuran metamorphosis.Vitamins and hormones · 2021Article
- Tools for experimental and computational analyses of off-target editing by programmable nucleases.Nature protocols · 2021Review
- Lysosomal storage diseases: current therapies and future alternatives.Journal of molecular medicine (Berlin, Germany) · 2020Review
- Rbm24 controls poly(A) tail length and translation efficiency ofProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Thyroid hormone activatesFrontiers in bioscience (Landmark edition) · 2020Article
- Genetic Deletion ofFrontiers in genetics · 2020Article
- Advancing genetic and genomic technologies deepen the pool for discovery in Xenopus tropicalis.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Review
- An Effective Microinjection Method and TALEN-Mediated Genome Editing in Pacific Abalone.Marine biotechnology (New York, N.Y.) · 2019Article
- Involvement of epigenetic modifications in thyroid hormone-dependent formation of adult intestinal stem cells during amphibian metamorphosis.General and comparative endocrinology · 2019Article
66 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 7 institutions in 2 countries.
Funding
Abstract
Transcription activator-like effector nucleases (TALENs) are an approach for directed gene disruption and have been proved to be effective in various animal models. Here, we report that TALENs can induce somatic mutations in Xenopus embryos with reliably high efficiency and that such mutations are heritable through germ-line transmission. We modified the Golden Gate method for TALEN assembly to make the product suitable for RNA transcription and microinjection into Xenopus embryos. Eight pairs of TALENs were constructed to target eight Xenopus genes, and all resulted in indel mutations with high efficiencies of up to 95.7% at the targeted loci. Furthermore, mutations induced by TALENs were highly efficiently passed through the germ line to F(1) frogs. Together with simple and reliable PCR-based approaches for detecting TALEN-induced mutations, our results indicate that TALENs are an effective tool for targeted gene editing/knockout in Xenopus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.