ArticleThe Journal of biological chemistry2012
Low activation and fast inactivation of transducin in carp cones.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- Mathematical analysis of phototransduction reaction parameters in rods and cones.Scientific reports · 2022Article
- Article
- Multi-scale, numerical modeling of spatio-temporal signaling in cone phototransduction.PloS one · 2019Article
- Regulation of calcium homeostasis in the outer segments of rod and cone photoreceptors.Progress in retinal and eye research · 2018Review
- Investigating the CaScientific reports · 2018Article
- The NaeLife · 2017Article
- Purification of cone outer segment for proteomic analysis on its membrane proteins in carp retina.PloS one · 2017Article
- The origin and evolution of cell types.Nature reviews. Genetics · 2016Review
- Dephosphorylation during bleach and regeneration of visual pigment in carp rod and cone membranes.The Journal of biological chemistry · 2015Article
- Phosphorylation-independent suppression of light-activated visual pigment by arrestin in carp rods and cones.The Journal of biological chemistry · 2015Article
- Activation and quenching of the phototransduction cascade in retinal cones as inferred from electrophysiology and mathematical modeling.Molecular vision · 2015Article
- Difference in the gain in the phototransduction cascade between rods and cones in carp.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Rod visual pigment optimizes active state to achieve efficient G protein activation as compared with cone visual pigments.The Journal of biological chemistry · 2014Article
- Quantitative aspects of cGMP phosphodiesterase activation in carp rods and cones.The Journal of biological chemistry · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cone photoreceptors show lower light sensitivity and briefer light responses than rod photoreceptors. The light detection signal in these cells is amplified through a phototransduction cascade. The first step of amplification in the cascade is the activation of a GTP-binding protein, transducin (Tr), by light-activated visual pigment (R*). We quantified transducin activation by measuring the binding of GTPγS in purified carp rod and cone membrane preparations with the use of a rapid quench apparatus and found that transducin activation by an R* molecule is ∼5 times less efficient in cones than in rods. Transducin activation terminated in less than 1 s in cones, more quickly than in rods. The rate of GTP hydrolysis in Tr*, and thus the rate of Tr* inactivation, was ∼25 times higher in cones than in rods. This faster inactivation of Tr* ensures briefer light responses in cones. The expression level of RGS9 was found to be ∼20 times higher in cones than in rods, which explains higher GTP hydrolytic activity and, thus, faster Tr* inactivation in cones than in rods. Although carp rods and cones express rod- or cone-versions of visual pigment and transducin, these molecules themselves do not seem to induce the differences significantly in the transducin activation and Tr* inactivation in rods and cones. Instead, the differences seem to be brought about in a rod or cone cell-type specific manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.